ArticleApplied and environmental microbiology2006
Contraselectable streptomycin susceptibility determinant for genetic manipulation and analysis of Helicobacter pylori.
Article in Applied and environmental microbiology, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
34 citing papers in PubMed, 43 citations in OpenAlex.
- Molecular insights into the fine-tuning of pH-dependent ArsR-mediated regulation of the SabA adhesin in Helicobacter pylori.Nucleic acids research · 2024Article
- RecombinantAntibiotics (Basel, Switzerland) · 2022Review
- Positive Selection of Mutations in the Helicobacter pyloriInfection and immunity · 2022Article
- TheMicroorganisms · 2021Article
- Small RNA mediated gradual control of lipopolysaccharide biosynthesis affects antibiotic resistance in Helicobacter pylori.Nature communications · 2021Article
- A genetic selection reveals functional metastable structures embedded in a toxin-encoding mRNA.eLife · 2019Article
- Complete genome sequence of Helicobacter pylori B128 7.13 and a single-step method for the generation of unmarked mutations.Helicobacter · 2019Article
- Role of a Stem-Loop Structure inInfection and immunity · 2019Article
- Identification of Genetic Features for Attenuation of TwoFrontiers in veterinary science · 2019Article
- Helicobacter pylori gene silencing in vivo demonstrates urease is essential for chronic infection.PLoS pathogens · 2017Article
- Application of FLP-FRT System to Construct Unmarked Deletion inFrontiers in microbiology · 2017Article
- Overcoming the Barrier of Low Efficiency during Genetic Transformation of Streptococcus mitis.Frontiers in microbiology · 2016Article
- Expansion of the tetracycline-dependent regulation toolbox for Helicobacter pylori.Applied and environmental microbiology · 2015Article
- Genes required for assembly of pili associated with the Helicobacter pylori cag type IV secretion system.Infection and immunity · 2014Article
- A repetitive DNA element regulates expression of the Helicobacter pylori sialic acid binding adhesin by a rheostat-like mechanism.PLoS pathogens · 2014Article
- Phase-variable restriction/modification systems are required for Helicobacter pylori colonization.Gut pathogens · 2014Article
- Development of a tetracycline-inducible gene expression system for the study of Helicobacter pylori pathogenesis.Applied and environmental microbiology · 2013Article
- Control of gene expression in Helicobacter pylori using the Tet repressor.Journal of microbiological methods · 2013Article
- Functional plasticity in the type IV secretion system of Helicobacter pylori.PLoS pathogens · 2013Article
- The sodium/proline transporter PutP of Helicobacter pylori.PloS one · 2013Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Many Helicobacter pylori genetic studies would benefit from an ability to move DNA sequences easily between strains by transformation and homologous recombination, without needing to leave a conventional drug resistance determinant at the targeted locus. Presented here is a two-gene cassette that can be selected both (i) against, due to a Campylobacter jejuni rpsL gene (dominant streptomycin susceptibility in cells also carrying an rpsL-str(r) allele), and (ii) for, due to an erm gene (erythromycin resistance). This rpsL,erm cassette's utility was assessed by using it to replace four gene loci (mdaB, frxA, fur, and nikR) in four streptomycin-resistant [Str(r)] strain backgrounds (derivatives of 26695, SS1, X47, and G27MA). The resultant 16 strains (phenotypically erythromycin resistant [Erm(r)] and Str(s)) were each transformed with wild-type genomic DNAs, and Str(r) derivatives were selected. The desired Erm(s) Str(r) isolates were obtained at frequencies that ranged from 17 to 96% among Str(r) transformants, with the Erm(s) yield apparently depending on the strain background and genome location of the targeted locus. The ease of isolating unmarked transformants described here should be valuable for many H. pylori molecular genetic and evolutionary analyses.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.