ArticleApplied and environmental microbiology2006
Rapid, sensitive, and discriminating identification of Naegleria spp. by real-time PCR and melting-curve analysis.
Article in Applied and environmental microbiology, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed.
- Noninvasive diagnostic biomarkers, genomic profiling, and advanced microscopic imaging in the early detection and characterization ofAnnals of medicine and surgery (2012) · 2024Review
- PCR Multiplexing Based on a Single Fluorescent Channel Using Dynamic Melting Curve Analysis.ACS omega · 2020Article
- A Fatal Case of Primary Amoebic Meningoencephalitis from Recreational Waters.Case reports in critical care · 2020Article
- Quantification of Malassezia pachydermatis by real-time PCR in swabs from the external ear canal of dogs.Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc · 2019Article
- Detection of contaminants in water supply: A review on state-of-the-art monitoring technologies and their applications.Sensors and actuators. B, Chemical · 2018Review
- Methodological approaches for monitoring opportunistic pathogens in premise plumbing: A review.Water research · 2017Review
- Naegleria fowleri after 50 years: is it a neglected pathogen?Journal of medical microbiology · 2016Article
- High-resolution melting analysis (HRM) for differentiation of four major Taeniidae species in dogs Taenia hydatigena, Taenia multiceps, Taenia ovis, and Echinococcus granulosus sensu stricto.Parasitology research · 2016Article
- Wide genetic variations at 18S ribosomal RNA locus of Cyclospora cayetanensis isolated from Egyptian patients using high resolution melting curve.Parasitology research · 2016Article
- Real-time PCR using FRET technology for Old World cutaneous leishmaniasis species differentiation.Parasites & vectors · 2016Article
- Predominance of Giardia lamblia assemblage A among iron deficiency anaemic pre-school Egyptian children.Parasitology research · 2016Article
- Detection of Schistosoma mansoni and Schistosoma haematobium by Real-Time PCR with High Resolution Melting Analysis.International journal of molecular sciences · 2015Article
- Comparison of real-time PCR methods for the detection of Naegleria fowleri in surface water and sediment.Parasitology research · 2015Article
- Article
- Differential Legionella spp. survival between intracellular and extracellular forms in thermal spring environments.Environmental science and pollution research international · 2013Article
- Quantitative detection and identification of Naegleria spp. in various environmental water samples using real-time quantitative PCR assay.Parasitology research · 2013Article
- Rapid detection and identification of human hookworm infections through high resolution melting (HRM) analysis.PloS one · 2012Article
- Genotypes of Giardia intestinalis clinical isolates of gastrointestinal symptomatic and asymptomatic Saudi children.Parasitology research · 2011Article
- Development of high-resolution melting (HRM) analysis for population studies of Fascioloides magna (Trematoda: Fasciolidae), the giant liver fluke of ruminants.Parasitology research · 2011Article
- Importance of nonenteric protozoan infections in immunocompromised people.Clinical microbiology reviews · 2010Review
Corrections and comments
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The free-living amoeboflagellate genus Naegleria includes one pathogenic and two potentially pathogenic species (Naegleria fowleri, Naegleria italica, and Naegleria australiensis) plus numerous benign organisms. Monitoring of bathing water, water supplies, and cooling systems for these pathogens requires a timely and reliable method for identification, but current DNA sequence-based methods identify only N. fowleri or require full sequencing to identify other species in the genus. A novel closed-tube method for distinguishing thermophilic Naegleria species is presented, using a single primer set and the DNA intercalating dye SYTO9 for real-time PCR and melting-curve analysis of the 5.8S ribosomal DNA gene and flanking noncoding spacers (ITS1, ITS2). Collection of DNA melting data at close temperature intervals produces highly informative melting curves with one or more recognizable melting peaks, readily distinguished for seven Naegleria species and the related Willaertia magna. Advantages over other methods used to identify these organisms include its comprehensiveness (encompassing all species tested to date), simplicity (no electrophoresis required to verify the product), and sensitivity (unambiguous identification from DNA equivalent to one cell). This approach should be applicable to a wide range of microorganisms of medical importance.
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