ArticleThe journal of histochemistry and cytochemistry : official journal of the Histochemistry Society2006
Lysosomal destabilization contributes to apoptosis of germinal center B-lymphocytes.
Article in The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 39 citations in OpenAlex.
- Underlying Mechanism of Lysosomal Membrane Permeabilization in CNS Injury: A Literature Review.Molecular neurobiology · 2025Review
- The Role of Cysteine Peptidases in Hematopoietic Stem Cell Differentiation and Modulation of Immune System Function.Frontiers in immunology · 2021Review
- Germinal center B cells selectively oxidize fatty acids for energy while conducting minimal glycolysis.Nature immunology · 2020Article
- The world is your oyster: low-dose, long-term microplastic exposure of juvenile oysters.Heliyon · 2020Article
- LRBA in the endomembrane system.Colombia medica (Cali, Colombia) · 2018Review
- PIG7 promotes leukemia cell chemosensitivity via lysosomal membrane permeabilization.Oncotarget · 2016Article
- GIMAP1 Is Essential for the Survival of Naive and Activated B Cells In Vivo.Journal of immunology (Baltimore, Md. : 1950) · 2016Article
- Deleterious mutations in LRBA are associated with a syndrome of immune deficiency and autoimmunity.American journal of human genetics · 2012Article
- Serglycin proteoglycan promotes apoptotic versus necrotic cell death in mast cells.The Journal of biological chemistry · 2012Article
- Article
- A role for serglycin proteoglycan in mast cell apoptosis induced by a secretory granule-mediated pathway.The Journal of biological chemistry · 2011Article
- Protein kinase C-δ isoform mediates lysosome labilization in DNA damage-induced apoptosis.International journal of oncology · 2011Article
- The surprising composition of the salivary proteome of preterm human newborn.Molecular & cellular proteomics : MCP · 2011Article
- Regulation of apoptosis-associated lysosomal membrane permeabilization.Apoptosis : an international journal on programmed cell death · 2010Review
- Endolysosomal proteases and their inhibitors in immunity.Nature reviews. Immunology · 2009Review
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
During germinal center (GC) reactions, B-lymphocytes with high-affinity B-cell receptors are selected. Regulation of apoptosis is a key process in selecting such wanted B-cells and in eliminating B-cells with unwanted specificities. In this paper, we show that apoptosis in human GC B-cells involves lysosomal destabilization, which is strictly controlled by caspase-8 activity, but not by caspase-9 activity. Ligation of CD40 provides resistance to lysosomal destabilization. Experimental lysosomal rupture by the lysosomotropic drug O-methyl-l-serine dodecylamide hydrochloride (MSDH) induces apoptosis in GC B-cells, including phosphatidyl serine exposure, mitochondrial inactivation, and DNA fragmentation. These apoptotic features occur in the absence of caspase-3 activity. Follicular dendritic cells (FDCs) protect binding B-lymphocytes from lysosomal destabilization, in both the absence and the presence of MSDH. Our study demonstrates that lysosomal leakage induces apoptosis of GC B-cells in a caspase-3-independent manner and that high-affinity binding to FDCsprevents lysosomal leakage and apoptosis in GC B-cells.
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Registered trials
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