ArticleThe British journal of ophthalmology2007
Expression of haematopoietic stem cell markers, CD133 and CD34 on human corneal keratocytes.
Article in The British journal of ophthalmology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 39 citations in OpenAlex.
- Corneal stromal mesenchymal stem cells: reconstructing a bioactive cornea and repairing the corneal limbus and stromal microenvironment.International journal of ophthalmology · 2021Review
- Fibroblastic and bone marrow-derived cellularity in the corneal stroma.Experimental eye research · 2021Article
- Article
- Postnatal periodontal ligament as a novel adult stem cell source for regenerative corneal cell therapy.Journal of cellular and molecular medicine · 2018Article
- Effect of Isolation Technique and Location on the Phenotype of Human Corneal Stroma-Derived Cells.Stem cells international · 2017Article
- Role of Human Corneal Stroma-Derived Mesenchymal-Like Stem Cells in Corneal Immunity and Wound Healing.Scientific reports · 2016Article
- Macromolecularly crowded in vitro microenvironments accelerate the production of extracellular matrix-rich supramolecular assemblies.Scientific reports · 2015Article
- Concise review: evidence for CD34 as a common marker for diverse progenitors.Stem cells (Dayton, Ohio) · 2014Review
- Keeping an eye on decellularized corneas: a review of methods, characterization and applications.Journal of functional biomaterials · 2013Article
- Characterization of corneal stromal stem cells with the potential for epithelial transdifferentiation.Stem cell research & therapy · 2013Article
- Using bovine pituitary extract to increase proliferation of keratocytes and maintain their phenotype in vitro.International journal of ophthalmology · 2013Article
- Therapeutic approaches to target cancer stem cells.Cancers · 2011Article
- Differential gene expression patterns of the developing and adult mouse cornea compared to the lens and tendon.Experimental eye research · 2008Article
- Increased Anti-Inflammatory Therapeutic Potential and Progenitor Marker Expression of Corneal Mesenchymal Stem Cells Cultured in an Optimized Propagation Medium.Cell transplantationArticle
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
aimTo study the expression of CD133 and CD34 antigens on cultured human keratocytes over time.
methodsPrimary cultures of human corneal stromal cells were established from explants derived from cadaver eye donors. The cultures were sorted for CD133+ and CD34+ cells using magnetic beads. Both the primary cultures and secondary passages of sorted cells were further analysed by flow cytometry and western blot analysis for expression of the same antigens over time.
resultsFour different cell populations-namely, CD133+, CD133-, CD34+ and CD34-, were identified in the culture samples. Two further specific subgroups were identified by flow cytometry: CD133+/CD34- cells and CD133+/CD34+ cells. Expression of CD133 declines more than CD34 with time in cell cultures. Although most cells lost expression of these markers, small populations retained staining up to 5 weeks in culture.
conclusionHuman keratocytes express the haematopoietic stem cell markers CD133 and CD34. This expression decreases with time in culture, with most but not all cells losing expression. On the basis of these markers, the corneal stroma shows a heterogeneous population of cells. Expression or down regulation of expression of these molecules could represent different stages of activation of these cells.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.