ArticleTraffic (Copenhagen, Denmark)2006
Regulatory dissociation of Tctex-1 light chain from dynein complex is essential for the apical delivery of rhodopsin.
Article in Traffic (Copenhagen, Denmark), 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
24 citing papers in PubMed, 39 citations in OpenAlex.
- Review
- Aurora A phosphorylates Ndel1 to reduce the levels of Mad1 and NuMA at spindle poles.Molecular biology of the cell · 2023Article
- The dynein light chain protein Tda2 functions as a dimerization engine to regulate actin capping protein during endocytosis.Molecular biology of the cell · 2021Article
- Tctex-1, a novel interaction partner of Kidney Injury Molecule-1, is required for efferocytosis.Journal of cellular physiology · 2018Article
- Proteomics Studies on the three Larval Stages of Development and Metamorphosis of Babylonia areolata.Scientific reports · 2018Article
- Desmosomal cadherin association with Tctex-1 and cortactin-Arp2/3 drives perijunctional actin polymerization to promote keratinocyte delamination.Nature communications · 2018Article
- Novel function of a dynein light chain in actin assembly during clathrin-mediated endocytosis.The Journal of cell biology · 2017Article
- Aberrant Expression of Dynein light chain 1 (DYNLT1) is Associated with Human Male Factor Infertility.Molecular & cellular proteomics : MCP · 2015Article
- Ins and outs of GPCR signaling in primary cilia.EMBO reports · 2015Review
- Cdc42 and sec10 Are Required for Normal Retinal Development in Zebrafish.Investigative ophthalmology & visual science · 2015Article
- Light regulates the ciliary protein transport and outer segment disc renewal of mammalian photoreceptors.Developmental cell · 2015Article
- Article
- Nucleotide bound to rab11a controls localization in rod cells but not interaction with rhodopsin.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2014Article
- Four-dimensional live imaging of apical biosynthetic trafficking reveals a post-Golgi sorting role of apical endosomal intermediates.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- Phosphorylation of DYNLT1 at serine 82 regulates microtubule stability and mitochondrial permeabilization in hypoxia.Molecules and cells · 2013Article
- Dlic1 deficiency impairs ciliogenesis of photoreceptors by destabilizing dynein.Cell research · 2013Article
- PtdIns(4)P regulates retromer-motor interaction to facilitate dynein-cargo dissociation at the trans-Golgi network.Nature cell biology · 2013Article
- Role of membrane traffic in the generation of epithelial cell asymmetry.Nature cell biology · 2012Review
- A role for Tctex-1 (DYNLT1) in controlling primary cilium length.European journal of cell biology · 2011Article
- Recruitment of dynein to late endosomes and lysosomes through light intermediate chains.Molecular biology of the cell · 2011Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
Post-Golgi to apical surface delivery in polarized epithelial cells requires the cytoplasmic dynein motor complex. However, the nature of dynein-cargo interactions and their underlying regulation are largely unknown. Previous studies have shown that the apical surface targeting of rhodopsin requires the dynein light chain, Tctex-1, which binds directly to both dynein intermediate chain (IC) and rhodopsin. In this report, we show that the S82E mutant of Tctex-1, which mimics Tctex-1 phosphorylated at serine 82, has a reduced affinity for dynein IC but not for rhodopsin. Velocity sedimentation experiments further suggest that S82E is not incorporated into the dynein complex. The dominant-negative effect of S82E causes rhodopsin mislocalization in polarized Madin-Darby canine kidney (MDCK) cells. The S82A mutant, which mimics dephosphorylated Tctex-1, can be incorporated into dynein complex but is impaired in its release. Expression of S82A also causes disruption of the apical localization of rhodopsin in MDCK cells. Taken together, these results suggest that the dynein complex disassembles to release cargo due to the specific phosphorylation of Tctex-1 at the S82 residue and that this process is critical for the apical delivery of membrane cargoes.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.