ArticleMolecular and cellular biology2006
Identification of new human origins of DNA replication by an origin-trapping assay.
Article in Molecular and cellular biology, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 22 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
22 citing papers in PubMed, 39 citations in OpenAlex.
- Episomes and Transposases-Utilities to Maintain Transgene Expression from Nonviral Vectors.Genes · 2022Review
- Article
- Involvement of G-quadruplex regions in mammalian replication origin activity.Nature communications · 2019Article
- The Mutator Phenotype: Adapting Microbial Evolution to Cancer Biology.Frontiers in genetics · 2019Review
- Regulation of the initiation of DNA replication in human cells.DNA repair · 2018Review
- Human DNA Helicase B as a Candidate for Unwinding Secondary CGG Repeat Structures at theFrontiers in molecular neuroscience · 2018Article
- Histone H4K20 tri-methylation at late-firing origins ensures timely heterochromatin replication.The EMBO journal · 2017Article
- Human DNA helicase B interacts with the replication initiation protein Cdc45 and facilitates Cdc45 binding onto chromatin.Experimental cell research · 2015Article
- Cis-acting DNA sequence at a replication origin promotes repeat expansion to fragile X full mutation.The Journal of cell biology · 2014Article
- Regulating DNA replication in eukarya.Cold Spring Harbor perspectives in biology · 2013Review
- The origin recognition complex in human diseases.Bioscience reports · 2013Review
- Open chromatin structures regulate the efficiencies of pre-RC formation and replication initiation in Epstein-Barr virus.The Journal of cell biology · 2012Article
- Phosphorylation of ORC2 protein dissociates origin recognition complex from chromatin and replication origins.The Journal of biological chemistry · 2012Article
- Chromatin remodeler sucrose nonfermenting 2 homolog (SNF2H) is recruited onto DNA replication origins through interaction with Cdc10 protein-dependent transcript 1 (Cdt1) and promotes pre-replication complex formation.The Journal of biological chemistry · 2011Article
- The dyad symmetry element of Epstein-Barr virus is a dominant but dispensable replication origin.PloS one · 2011Article
- Why are we where we are? Understanding replication origins and initiation sites in eukaryotes using ChIP-approaches.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2010Article
- Differential targeting of Tetrahymena ORC to ribosomal DNA and non-rDNA replication origins.The EMBO journal · 2009Article
- RNA-dependent recruitment of the origin recognition complex.The EMBO journal · 2008Article
- Conditional gene vectors regulated in cis.Nucleic acids research · 2008Article
- One-way PCR-based mapping of a replication initiation point (RIP).Nature protocols · 2008Article
Corrections and comments
- Erratum issued
Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Metazoan genomes contain thousands of replication origins, but only a limited number have been characterized so far. We developed a two-step origin-trapping assay in which human chromatin fragments associated with origin recognition complex (ORC) in vivo were first enriched by chromatin immunoprecipitation. In a second step, these fragments were screened for transient replication competence in a plasmid-based assay utilizing the Epstein-Barr virus latent origin oriP. oriP contains two elements, an origin (dyad symmetry element [DS]) and the family of repeats, that when associated with the viral protein EBNA1 facilitate extrachromosomal stability. Insertion of the ORC-binding human DNA fragments in oriP plasmids in place of DS enabled us to screen functionally for their abilities to restore replication. Using the origin-trapping assay, we isolated and characterized five previously unknown human origins. The assay was validated with nascent strand abundance assays that confirm these origins as active initiation sites in their native chromosomal contexts. Furthermore, ORC and MCM2-7 components localized at these origins during G(1) phase of the cell cycle but were not detected during mitosis. This finding extends the current understanding of origin-ORC dynamics by suggesting that replication origins must be reestablished during the early stages of each cell division cycle and that ORC itself participates in this process.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.