ArticleJournal of clinical microbiology2006
Real-time PCR assay for detection and quantification of hepatitis B virus genotypes A to G.
Article in Journal of clinical microbiology, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
25 citing papers in PubMed.
- Inhibition of Hepatitis B Virus Replication by a Novel GalNAc-siRNA In Vivo and In Vitro.ACS omega · 2025Article
- ELISA genotyping of hepatitis B virus in China with antibodies specific for genotypes B and C.Scientific reports · 2024Article
- Article
- Simple Low-Cost Production of DNA MS2 Virus-Like Particles As Molecular Diagnostic Controls.GEN biotechnology · 2022Article
- Recombinase amplified CRISPR enhanced chain reaction (RACECAR) for viral genome detection.Nanoscale · 2022Article
- More DNA and RNA of HBV SP1 splice variants are detected in genotypes B and C at low viral replication.Scientific reports · 2021Article
- Molecular Detection of Epstein-Barr Virus, Human Herpes Virus 6, Cytomegalovirus, and Hepatitis B Virus in Patients with Multiple Sclerosis.Middle East journal of digestive diseases · 2020Article
- Review-Chemical and Biological Sensors for Viral Detection.Journal of the Electrochemical Society · 2020Article
- Amplification chemistries in clinical virology.Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology · 2019Review
- Copy number-based quantification assay for non-invasive detection of PVT1-derived transcripts.PloS one · 2019Article
- Hepatocytic expression of human sodium-taurocholate cotransporting polypeptide enables hepatitis B virus infection of macaques.Nature communications · 2017Article
- Recent Advances in Nanoparticle Concentration and Their Application in Viral Detection Using Integrated Sensors.Sensors (Basel, Switzerland) · 2017Review
- Development and Validation of a Novel Real-time Assay for the Detection and Quantification ofFrontiers in public health · 2017Article
- Article
- An in-house real-time polymerase chain reaction: standardisation and comparison with the Cobas Amplicor HBV monitor and Cobas AmpliPrep/Cobas TaqMan HBV tests for the quantification of hepatitis B virus DNA.Memorias do Instituto Oswaldo Cruz · 2016Article
- Detection of hepatitis B virus infection: A systematic review.World journal of hepatology · 2015Article
- Dynamic comparison between Daan real-time PCR and Cobas TaqMan for quantification of HBV DNA levels in patients with CHB.BMC infectious diseases · 2014Article
- Hepatitis B virus DNA splicing in Lebanese blood donors and genotype A to E strains: implications for hepatitis B virus DNA quantification and infectivity.Journal of clinical microbiology · 2012Article
- A latest and promising approach for prediction of viral load in hepatitis B virus infected patients.Indian journal of human genetics · 2011Article
- Validation of laboratory-developed molecular assays for infectious diseases.Clinical microbiology reviews · 2010Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors.
Funding
Abstract
The detection and quantification of hepatitis B virus (HBV) DNA play an important role in diagnosing and monitoring HBV infection as well as assessing therapeutic response. The great variability among HBV genotypes and the enormous range of clinical HBV DNA levels present challenges for PCR-based amplification techniques. In this study, we describe the development, evaluation, and validation of a novel real-time PCR assay designed to provide accurate quantification of DNA from all eight HBV genotypes in patient plasma specimens. A computer algorithm was used to design degenerate real-time PCR primers and probes based upon a large number (n = 340) of full-length genomic sequences including HBV genotypes A to H from Europe, Africa, Asia, and North and South America. Genotype performance was tested and confirmed using 59 genotype A to G specimens from two commercially available worldwide genotype panels. This assay has a dynamic range of at least 8 log(10) without the need for specimen dilution, good clinical intra- and interassay precision, and excellent correlation with the Bayer Diagnostics VERSANT HBV DNA 3.0 (branched DNA) assay (r = 0.93). Probit analysis determined the 95% detection level was 56 IU/ml, corresponding to 11 copies per PCR well. The high sensitivity, wide linear range, good reproducibility, and genotype inclusivity, combined with a small sample volume requirement and low cost, make this novel quantitative HBV real-time PCR assay particularly well suited for application to large clinical and epidemiological studies.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.