ArticleJournal of clinical microbiology2006
High-throughput identification of clinical pathogenic fungi by hybridization to an oligonucleotide microarray.
Article in Journal of clinical microbiology, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed.
- Multiplex size marker (YEAST PLEX) for rapid and accurate identification of pathogenic yeasts.Journal of clinical laboratory analysis · 2022Article
- Soil amendment in plastic greenhouse using modified biochar: soil bacterial diversity responses and microbial biomass carbon and nitrogen.Biotechnology letters · 2021Article
- Visible DNA microarray and loop-mediated isothermal amplification (LAMP) for the identification of Cryptococcus species recovered from culture medium and cerebrospinal fluid of patients with meningitis.Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologica · 2020Article
- Development and Assessment of a Diagnostic DNA Oligonucleotide Microarray for Detection and Typing of Meningitis-Associated Bacterial Species.High-throughput · 2018Article
- Visible DNA Microarray System as an Adjunctive Molecular Test in Identification of Pathogenic Fungi Directly from a Blood Culture Bottle.Journal of clinical microbiology · 2018Article
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- Diseases and Molecular Diagnostics: A Step Closer to Precision Medicine.Indian journal of clinical biochemistry : IJCB · 2017Article
- Molecular variation analysis ofExperimental and therapeutic medicine · 2016Article
- Molecular characterization of the Indian poultry nodular tapeworm, Raillietina echinobothrida (Cestoda: Cyclophyllidea: Davaineidae) based on rDNA internal transcribed spacer 2 region.Journal of parasitic diseases : official organ of the Indian Society for Parasitology · 2014Article
- Visual analysis of DNA microarray data for accurate molecular identification of non-albicans Candida isolates from patients with candidemia episodes.Journal of clinical microbiology · 2013Article
- Article
- Candida infections and their prevention.ISRN preventive medicine · 2013Review
- Evidence of melanoma in wild marine fish populations.PloS one · 2012Article
- Rapid identification of allergenic and pathogenic molds in environmental air by an oligonucleotide array.BMC infectious diseases · 2011Article
- Basic concepts of microarrays and potential applications in clinical microbiology.Clinical microbiology reviews · 2009Review
- Characterization of microbial pathogens by DNA microarrays.Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases · 2009Review
- Colony multiplex-tandem PCR for rapid, accurate identification of fungal cultures.Journal of clinical microbiology · 2008Article
- Practical method for detection and identification of Candida, Aspergillus, and Scedosporium spp. by use of rolling-circle amplification.Journal of clinical microbiology · 2008Article
- Rapid identification and differentiation of Trichophyton species, based on sequence polymorphisms of the ribosomal internal transcribed spacer regions, by rolling-circle amplification.Journal of clinical microbiology · 2008Article
- DNA microarray based on arrayed-primer extension technique for identification of pathogenic fungi responsible for invasive and superficial mycoses.Journal of clinical microbiology · 2008Article
Corrections and comments
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Here we report the development of an oligonucleotide microarray method that can identify fungal pathogens in a single reaction. Specific oligonucleotide probes targeted to internal transcribed spacer 2 were designed and synthesized. Fungal DNA was amplified by universal primers, and the PCR product was hybridized with the oligonucleotide microarray. A series of specific hybridization profiles corresponding to species were obtained. The 122 strains of fungal pathogens, including standard and clinically isolated strains, used to test the specificity, stability, and sensitivity of the microarray system belonged to 20 species representing 8 genera. We found that the microarray system can successfully discriminate among the fungal pathogens to the species level, with high specificity and stability. The sensitivity was 15 pg/ml of DNA. This oligonucleotide microarray system represents a rapid, simple, and reliable alternative to conventional methods of identifying common clinical fungal isolates.
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