ArticleJournal of clinical microbiology2006
Rapid and sensitive detection of single cryptosporidium oocysts from archived glass slides.
Article in Journal of clinical microbiology, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
7 citing papers in PubMed.
- A rapid and sensitive system for recovery of nucleic acids from Mycobacteria sp. on archived glass slides.BMC microbiology · 2018Article
- Prevalence ofElectronic physician · 2017Article
- Real-time PCR assay targeting the actin gene for the detection of Cryptosporidium parvum in calf fecal samples.Parasitology research · 2012Article
- Gram stains: a resource for retrospective analysis of bacterial pathogens in clinical studies.PloS one · 2012Article
- Unexpected results from large-scale cryptosporidiosis screening study in calves in Tanzania.Annals of tropical medicine and parasitology · 2011Article
- Occurrence of Giardia and Cryptosporidium in Italian water supplies.Environmental monitoring and assessment · 2009Article
- Effectiveness of standard UV depuration at inactivating Cryptosporidium parvum recovered from spiked Pacific oysters (Crassostrea gigas).Applied and environmental microbiology · 2007Article
Corrections and comments
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Authors and funding
8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In this study we report on the development and application of a novel method for efficiently extracting and detecting single Cryptosporidium oocysts from archived glass slides. Laser capture microscopy was used to extract low numbers of oocysts from archived glass slides. Highly sensitive real-time PCR methods were then developed to enable the rapid detection and identification of Cryptosporidium oocysts from these samples. The method was applied to fecal smears stained with a variety of standard oocyst stains and water samples. This application, with samples derived from both public health and water service laboratories, highlighted the strong potential of this method to be used as a rapid high-throughput screening tool for the routine monitoring of Cryptosporidium and other medically important pathogens from clinical, veterinary, and environmental water samples. Importantly, the application of our protocol could be used to type Cryptosporidium and other pathogens from stored archived glass slides in public health and water service laboratories, providing vital epidemiological updates and helping to identify and trace pathogens and their routes of infection and ultimately improve their control.
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Registered trials
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