ArticleJournal of clinical microbiology2006
Real-time reverse transcription-PCR for detection of rotavirus and adenovirus as causative agents of acute viral gastroenteritis in children.
Article in Journal of clinical microbiology, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 65 papers, 2 of them syntheses that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
65 citing papers in PubMed, 2 syntheses or guidelines pooled it, 194 citations in OpenAlex.
- Systematic review of point-of-need molecular diagnostics for rotavirus and enteric adenoviruses F40/F41.BMC infectious diseases · 2026Pooled it
- Human adenoviruses in children with gastroenteritis: a systematic review and meta-analysis.BMC infectious diseases · 2024Pooled it
- No evidence of zoonotic viruses in selected captive and wild-caught grasscutters (Thryonomys swinderianus) in the ashanti region of ghana: a cross-sectional study.BMC veterinary research · 2026Article
- Evaluation of different genomic regions of rotavirus B and rotavirus C for development of real-time RT‒PCR assays.Virology journal · 2024Article
- Foodborne Viruses and Somatic Coliphages Occurrence in Fresh Produce at Retail from Northern Mexico.Food and environmental virology · 2024Article
- Adenovirus is Not Detected in Liver Tissue From a Historical Multicenter Cohort of Children With Acute Liver Failure.Journal of pediatric gastroenterology and nutrition · 2023Article
- Synergistic Effects of Rotavirus and Co-Infecting Viral Enteric Pathogens on Diarrheal Disease - Guangzhou City, Guangdong Province, China, 2019.China CDC weekly · 2023Article
- Influence of herd immunity on norovirus: a long-term field study of repeated viral gastroenteritis outbreaks at the same facilities.BMC infectious diseases · 2023Article
- Molecular Prevalence of Diarrheal Pathogens in Children with Acute Diarrhea in Southeastern Gabon.The American journal of tropical medicine and hygiene · 2023Article
- Canine Distemper Virus in Autochtonous and Imported Dogs, Southern Italy (2014-2021).Animals : an open access journal from MDPI · 2022Article
- First report of canine bufavirus in India.Archives of virology · 2022Article
- Development of a Real-Time Reverse Transcription-PCR Assay To Detect and Quantify Group A Rotavirus Equine-Like G3 Strains.Journal of clinical microbiology · 2021Article
- Detection and evaluation of rotavirus surveillance methods as viral indicator in the aquatic environments.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2021Review
- Validation of a Laboratory-Developed Triplex Molecular Assay for Simultaneous Detection of Gastrointestinal Adenovirus and Rotavirus in Stool Specimens.Pathogens (Basel, Switzerland) · 2020Article
- Lifestyle as Risk Factor for Infectious Causes of Death in Young Dogs: A Retrospective Study in Southern Italy (2015-2017).Veterinary medicine international · 2020Article
- Children Attending Day Care Centers are a Year-round Reservoir of Gastrointestinal Viruses.Scientific reports · 2019Observational
- Comparison of three multiplex real-time PCR assays for detection of enteric viruses in patients with diarrhea.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2019Article
- Virus detection by transmission electron microscopy: Still useful for diagnosis and a plus for biosafety.Reviews in medical virology · 2019Review
- Molecular epidemiology of human adenovirus infections in Denmark, 2011-2016.Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology · 2018Article
- No evidence of enteric viral involvement in the new neonatal porcine diarrhoea syndrome in Danish pigs.BMC veterinary research · 2017Article
5 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Viral pathogens are the most common cause of gastroenteritis in developed countries. Human rotavirus and adenovirus infections are major causes of acute outbreaks and sporadic cases of gastroenteritis, occurring primarily among children less than 2 years of age. Patient hospitalization is often required, with enormous infection control implications. This work describes the development of real-time PCR assays for the detection of group F adenovirus, rotavirus A, and rotavirus C from stool specimens. Two hundred twenty stool samples from pediatric patients exhibiting symptoms of diarrhea and/or vomiting were examined. PCR results were compared with those of virus detection by electron microscopy and latex agglutination antigen detection. The incorporation of an internal-control RNA that was spiked into individual stool extracts functioned as an internal validation for the reporting of PCR-negative results. Rotavirus C was not detected by real-time PCR in the patient stool samples examined. Real-time reverse transcription-PCR resulted in 175% and 111% increases in the rates of detection of adenovirus F and rotavirus A, respectively, compared with latex agglutination testing. Molecular detection increased the number of stool specimens in which causative agents of gastroenteritis were identified by 155% compared to electron microscopy. Genotyping of a proportion of the rotavirus and adenovirus strains identified only genotype G1 rotavirus and both adenovirus genotypes 40 and 41 in circulation within the patient cohort examined. The results highlight the significance of rapid molecular methods for the routine screening of stool samples in hospital laboratories to provide rapid definitive diagnoses.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.