ArticleJournal of clinical microbiology2006
Evaluation of real-time and conventional PCR targeting complex 85 genes for detection of Mycobacterium leprae DNA in skin biopsy samples from patients diagnosed with leprosy.
Article in Journal of clinical microbiology, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 41 papers, 1 of them a synthesis that pooled it.
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Who cites it
41 citing papers in PubMed, 1 synthesis or guideline pooled it, 105 citations in OpenAlex.
- Comparative analysis of the leprosy detection rate regarding its clinical spectrum through PCR using theFrontiers in microbiology · 2024Pooled it
- Dual template (epitope) imprinting of Mycobacterium leprae bacterial protein for enhanced MIP-EQCM sensing.Analytical and bioanalytical chemistry · 2026Article
- Molecular Detection ofTropical medicine and infectious disease · 2025Review
- A comparative study on the detection of Mycobacterium leprae DNA in urine samples of leprosy patients using Rlep-PCR with other conventional samples.Molecular biology reports · 2024Article
- Target product profiles: leprosy diagnostics.Bulletin of the World Health Organization · 2024Article
- Quantitative Real-Time Polymerase Chain Reaction for Detection of Mycobacterium leprae DNA in Tissue Specimens from Patients with Leprosy.The American journal of tropical medicine and hygiene · 2023Article
- Development and validation of a multiplex real-time qPCR assay using GMP-grade reagents for leprosy diagnosis.PLoS neglected tropical diseases · 2022Article
- Heterogeneous persistence of Mycobacterium leprae in oral and nasal mucosa of multibacillary patients during multidrug therapy.Memorias do Instituto Oswaldo Cruz · 2022Article
- Leprosy: A Review of Epidemiology, Clinical Diagnosis, and Management.Journal of tropical medicine · 2022Review
- Utility of Multi-target Nested PCR and ELISPOT Assays for the Detection of Paucibacillary Leprosy: A Possible Conclusion of Clinical Laboratory Misdiagnosis.Frontiers in cellular and infection microbiology · 2022Article
- RLEP LAMP for the laboratory confirmation of leprosy: towards a point-of-care test.BMC infectious diseases · 2021Article
- Evolutionary history ofPhilosophical transactions of the Royal Society of London. Series B, Biological sciences · 2020Article
- Ultra-sensitive detection of Mycobacterium leprae: DNA extraction and PCR assays.PLoS neglected tropical diseases · 2020Article
- Molecular detection ofMolecular biology research communications · 2020Article
- Prospects for new leprosy diagnostic tools, a narrative review considering ELISA and PCR assays.Revista da Sociedade Brasileira de Medicina Tropical · 2020Review
- Develop and Field Evolution of Single Tube Nested PCR, SYBRGreen PCR Methods, for the Diagnosis of Leprosy in Paraffin-embedded Formalin Fixed Tissues in Yunnan Province, a Hyper endemic Area of Leprosy in China.PLoS neglected tropical diseases · 2019Article
- Development of a combined RLEP/16S rRNA (RT) qPCR assay for the detection of viable M. leprae from nasal swab samples.BMC infectious diseases · 2019Article
- A novel integrated molecular and serological analysis method to predict new cases of leprosy amongst household contacts.PLoS neglected tropical diseases · 2019Article
- Development and evaluation of a droplet digital PCR assay for the diagnosis of paucibacillary leprosy in skin biopsy specimens.PLoS neglected tropical diseases · 2019Article
- Quantitative polymerase chain reaction in paucibacillary leprosy diagnosis: A follow-up study.PLoS neglected tropical diseases · 2019Article
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In spite of the decrease in the number of registered leprosy patients, the number of new cases diagnosed each year (400,000) has remained essentially unchanged. Leprosy diagnosis is difficult due to the low sensitivity of current methodologies to identify new cases. In this study, conventional and TaqMan real-time PCR assays for detection of Mycobacterium leprae DNA were compared to current classification based on clinical, bacteriological, and histological evaluation. M. leprae DNA was extracted from frozen skin biopsy specimens from 69 leprosy patients enrolled in the study and was amplified using specific primers for either the antigen 85B-coding gene or the 85A-C intergenic region by using conventional and real-time PCR. The detection rate was 100% among multibacillary (MB) patients and ranged from 62.5% to 79.2% among paucibacillary (PB) patients according to the assay used. The TaqMan system for 85B gene amplification showed the highest sensitivity, although conventional PCR using the 85A-C gene as a target was also efficient. The cycle threshold (C(T)) values obtained using the TaqMan system were able to statistically (P < 0.0001) differentiate MB (mean C(T), 28.06; standard deviation [SD], 4.51) from PB (mean C(T), 33.06; SD, 2.24) patients. Also, there was a correlation between C(T) values and the bacteriological index for MB patients (Pearson's r, -0.444; P = 0.008). Within the PB patients' group, we tested normal skin from six patients exhibiting the pure neuritic form of leprosy (PNL). Five out of six PNL patients were positive for the presence of M. leprae DNA, even in the absence of skin lesions. In conclusion, the TaqMan real-time PCR developed here seems to be a useful tool for rapidly detecting and quantifying M. leprae DNA in clinical specimens in which bacilli were undetectable by conventional histological staining.
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