ArticleJournal of clinical microbiology2006
Development of an internal positive control for rapid diagnosis of avian influenza virus infections by real-time reverse transcription-PCR with lyophilized reagents.
Article in Journal of clinical microbiology, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
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Who cites it
35 citing papers in PubMed, 99 citations in OpenAlex.
- Development of a new TaqMan real-time PCR for detecting Psittacine adenovirus 1 (PsAdV-1).BMC veterinary research · 2026Article
- Validation and multi-site deployment of a lyophilized qRT-PCR reagent for the molecular diagnosis of avian influenza and rabies in Sub-Saharan African regions.Journal of clinical microbiology · 2025Article
- Randomized-field microwave-assisted pharmaceutical lyophilization with closed-loop control.Scientific reports · 2025Article
- Optimization and Benchmarking of RT-LAMP-CRISPR-Cas12a for the Detection of SARS-CoV-2 in Saliva.International journal of molecular sciences · 2025Article
- Avian Influenza Screening in Captive Wild Birds and Biosecurity Appraisal of Zoological Gardens in Southwestern Nigeria.Veterinary medicine international · 2025Article
- Enhanced Recovery and Detection of Highly Infectious Animal Disease Viruses by Virus Capture Using NanotrapViruses · 2024Article
- Review
- Risk profile of low pathogenicity avian influenza virus infections in farms in southern Vietnam.The Journal of veterinary medical science · 2022Article
- Assessment of Successful qRT-PCR of SARS-CoV-2 Assay in Pool Screening Using Isopropyl Alcohol Purification Step in RNA Extraction.BioMed research international · 2021Article
- Development and Evaluation of a Novel Armored RNA Technology Using Bacteriophage Qβ.Food and environmental virology · 2019Article
- A novel delivery platform based on Bacteriophage MS2 virus-like particles.Virus research · 2016Review
- Comparison of culture versus quantitative real-time polymerase chain reaction for the detection of Taylorella equigenitalis in field samples from naturally infected horses in Canada and Germany.Canadian journal of veterinary research = Revue canadienne de recherche veterinaire · 2015Article
- Designing novel and simple competitive internal amplification control for reliable PCR diagnosis of herpes simplex virus.Jundishapur journal of microbiology · 2015Article
- Sample-ready multiplex qPCR assay for detection of malaria.Malaria journal · 2014Article
- High genetic diversity of Newcastle disease virus in poultry in West and Central Africa: cocirculation of genotype XIV and newly defined genotypes XVII and XVIII.Journal of clinical microbiology · 2013Article
- Evaluation of the pooling of swabs for real-time PCR detection of low titre shedding of low pathogenicity avian influenza in turkeys.Epidemiology and infection · 2013Article
- An overview of the highly pathogenic H5N1 influenza virus.Virologica Sinica · 2013Review
- Detection of avian influenza viruses from shorebirds: evaluation of surveillance and testing approaches.Journal of wildlife diseases · 2012Article
- Article
- An optimized real-time PCR to avoid species-/tissue-associated inhibition for H5N1 detection in ferret and monkey tissues.TheScientificWorldJournal · 2012Article
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We developed an internal positive control (IPC) RNA to help ensure the accuracy of the detection of avian influenza virus (AIV) RNA by reverse transcription (RT)-PCR and real-time RT-PCR (RRT-PCR). The IPC was designed to have the same binding sites for the forward and reverse primers of the AIV matrix gene as the target amplicon, but it had a unique internal sequence used for the probe site. The amplification of the viral RNA and the IPC by RRT-PCR were monitored with two different fluorescent probes in a multiplex format, one specific for the AIV matrix gene and the other for the IPC. The RRT-PCR test was further simplified with the use of lyophilized bead reagents for the detection of AIV RNA. The RRT-PCR with the bead reagents was more sensitive than the conventional wet reagents for the detection of AIV RNA. The IPC-based RRT-PCR detected inhibitors in blood, kidney, lungs, spleen, intestine, and cloacal swabs, but not allantoic fluid, serum, or tracheal swabs The accuracy of RRT-PCR test results with the lyophilized beads was tested on cloacal and tracheal swabs from experimental birds inoculated with AIV and compared with virus isolation (VI) on embryonating chicken eggs. There was 97 to 100% agreement of the RRT-PCR test results with VI for tracheal swabs and 81% agreement with VI for cloacal swabs, indicating a high level of accuracy of the RRT-PCR assay. The same IPC in the form of armored RNA was also used to monitor the extraction of viral RNA and subsequent detection by RRT-PCR.
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