ArticleBMC genomics2006
Genome sequence alterations detected upon passage of Burkholderia mallei ATCC 23344 in culture and in mammalian hosts.
Article in BMC genomics, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed.
- Glanders: Historical military use and potential bioterrorism concern.Open veterinary journal · 2025Review
- Galleria mellonella-intracellular bacteria pathogen infection models: the ins and outs.FEMS microbiology reviews · 2023Review
- Influence of genomic variations on glanders serodiagnostic antigens using integrative genomic and transcriptomic approaches.Frontiers in veterinary science · 2023Article
- Sequencing-Based Genotyping of PakistaniPathogens (Basel, Switzerland) · 2022Article
- Genetic diversity and spatial distribution of Burkholderia mallei by core genome-based multilocus sequence typing analysis.PloS one · 2022Article
- 16S rDNA and ITS Sequence Diversity of Burkholderia mallei Isolated from Glanders-Affected Horses and Mules in India (2013-2019).Current microbiology · 2021Article
- In vitro passage alters virulence, immune activation and proteomic profiles of Burkholderia pseudomallei.Scientific reports · 2020Article
- Antibodies againstInfection and immunity · 2017Article
- Within-Host Evolution ofmBio · 2017Article
- Characterization of in vitro phenotypes of Burkholderia pseudomallei and Burkholderia mallei strains potentially associated with persistent infection in mice.Archives of microbiology · 2017Article
- Genome Sequence of the Historical Clinical Isolate Burkholderia pseudomallei PHLS 6.Genome announcements · 2016Article
- Twenty years of bacterial genome sequencing.Nature reviews. Microbiology · 2015Review
- The Autotransporter BpaB Contributes to the Virulence of Burkholderia mallei in an Aerosol Model of Infection.PloS one · 2015Article
- Virulence of Burkholderia mallei quorum-sensing mutants.Infection and immunity · 2013Article
- The early stage of bacterial genome-reductive evolution in the host.PLoS pathogens · 2010Article
- Article
- Critical protective role for MCP-1 in pneumonic Burkholderia mallei infection.Journal of immunology (Baltimore, Md. : 1950) · 2010Article
- Protection from pneumonic infection with burkholderia species by inhalational immunotherapy.Infection and immunity · 2009Article
- Strategies toward vaccines against Burkholderia mallei and Burkholderia pseudomallei.Expert review of vaccines · 2008Review
- Tandem repeat regions within the Burkholderia pseudomallei genome and their application for high resolution genotyping.BMC microbiology · 2007Article
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Authors and funding
9 authors.
Funding
Abstract
backgroundMore than 12,000 simple sequence repeats (SSRs) have been identified in the genome of Burkholderia mallei ATCC 23344. As a demonstrated mechanism of phase variation in other pathogenic bacteria, these may function as mutable loci leading to altered protein expression or structure variation. To determine if such alterations are occurring in vivo, the genomes of various single-colony passaged B. mallei ATCC 23344 isolates, one from each source, were sequenced from culture, a mouse, a horse, and two isolates from a single human patient, and the sequence compared to the published B. mallei ATCC 23344 genome sequence.
resultsForty-nine insertions and deletions (indels) were detected at SSRs in the five passaged strains, a majority of which (67.3%) were located within noncoding areas, suggesting that such regions are more tolerant of sequence alterations. Expression profiling of the two human passaged isolates compared to the strain before passage revealed alterations in the mRNA levels of multiple genes when grown in culture.
conclusionThese data support the notion that genome variability upon passage is a feature of B. mallei ATCC23344, and that within a host B. mallei generates a diverse population of clones that accumulate genome sequence variation at SSR and other loci.
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