ArticleJournal of bacteriology2006
Thiamine is synthesized by a salvage pathway in Rhizobium leguminosarum bv. viciae strain 3841.
Article in Journal of bacteriology, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed, 52 citations in OpenAlex.
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- Article
- Lifestyle adaptations ofProceedings of the National Academy of Sciences of the United States of America · 2020Article
- The ThiL enzyme is a valid antibacterial target essential for both thiamine biosynthesis and salvage pathways inThe Journal of biological chemistry · 2020Article
- A GMC Oxidoreductase GmcA Is Required for Symbiotic Nitrogen Fixation inFrontiers in microbiology · 2020Article
- Glutathione is Involved in Detoxification of Peroxide and Root Nodule Symbiosis of Mesorhizobium huakuii.Current microbiology · 2020Article
- Multilayered horizontal operon transfers from bacteria reconstruct a thiamine salvage pathway in yeasts.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- Phenotypic characterization of rhizobia nodulating legumesJournal of advanced research · 2018Article
- Thiaminase I Provides a Growth Advantage by Salvaging Precursors from Environmental Thiamine and Its Analogs in Burkholderia thailandensis.Applied and environmental microbiology · 2018Article
- Sequential induction of three recombination directionality factors directs assembly of tripartite integrative and conjugative elements.PLoS genetics · 2018Article
- Glutathione affects the transport activity of Rhizobium leguminosarum 3841 and is essential for efficient nodulation.FEMS microbiology letters · 2017Article
- Genomic insights into the thiamin metabolism ofStandards in genomic sciences · 2017Article
- Flavoenzyme CrmK-mediated substrate recycling in caerulomycin biosynthesis.Chemical science · 2016Article
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In the absence of added thiamine, Rhizobium leguminosarum bv. viciae strain 3841 does not grow in liquid medium and forms only "pin" colonies on agar plates, which contrasts with the good growth of Sinorhizobium meliloti 1021, Mesorhizobium loti 303099, and Rhizobium etli CFN42. These last three organisms have thiCOGE genes, which are essential for de novo thiamine synthesis. While R. leguminosarum bv. viciae 3841 lacks thiCOGE, it does have thiMED. Mutation of thiM prevented formation of pin colonies on agar plates lacking added thiamine, suggesting thiamine intermediates are normally present. The putative functions of ThiM, ThiE, and ThiD are 4-methyl-5-(beta-hydroxyethyl) thiazole (THZ) kinase, thiamine phosphate pyrophosphorylase, and 4-amino-5-hydroxymethyl-2-methyl pyrimidine (HMP) kinase, respectively. This suggests that a salvage pathway operates in R. leguminosarum, and addition of HMP and THZ enabled growth at the same rate as that enabled by thiamine in strain 3841 but elicited no growth in the thiM mutant (RU2459). There is a putative thi box sequence immediately upstream of the thiM, and a gfp-mut3.1 fusion to it revealed the presence of a promoter that is strongly repressed by thiamine. Using fluorescent microscopy and quantitative reverse transcription-PCR, it was shown that thiM is expressed in the rhizosphere of vetch and pea plants, indicating limitation for thiamine. Pea plants infected by RU2459 were not impaired in nodulation or nitrogen fixation. However, colonization of the pea rhizosphere by the thiM mutant was impaired relative to that of the wild type. Overall, the results show that a thiamine salvage pathway operates to enable growth of Rhizobium leguminosarum in the rhizosphere, allowing its survival when thiamine is limiting.
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