ArticleJournal of bacteriology2006
Functional characterization of IS1999, an IS4 family element involved in mobilization and expression of beta-lactam resistance genes.
Article in Journal of bacteriology, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 70 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
70 citing papers in PubMed, 130 citations in OpenAlex.
- Wildlife as a reservoir of OXA-48-like carbapenemase-producing Enterobacterales.Microbiology spectrum · 2026Article
- Examining the Roles of Genomic Context and Endogenous Regulatory Elements on ISInternational journal of molecular sciences · 2025Article
- Article
- The latent cis-regulatory potential of mobile DNA in Escherichia coli.Nature communications · 2025Article
- Giant transposons promote strain heterogeneity in a major fungal pathogen.bioRxiv : the preprint server for biology · 2025Article
- Microplastics and antibiotic resistance genes as rising threats: Their interaction represents an urgent environmental concern.Current research in microbial sciences · 2025Review
- Investigating How Genomic Contexts Impact IS5 Transposition Within theMicroorganisms · 2024Article
- Identification of an ArgR-controlled promoter within the outermost region of the ISJournal of bacteriology · 2024Article
- Comparison of Carbapenemases and Extended-Spectrum β-Lactamases and Resistance Phenotypes in Hospital- and Community-Acquired Isolates ofMicroorganisms · 2024Article
- Plasmid genomic epidemiology of carbapenem-hydrolysing class D β-lactamase (CDHL)-producingMicrobial genomics · 2024Article
- Role of amino acid 159 in carbapenem and temocillin hydrolysis of OXA-933, a novel OXA-48 variant.Antimicrobial agents and chemotherapy · 2024Article
- Antibiotic Susceptibility Profiles of Bacterial Isolates Recovered from Abscesses in Cattle and Sheep at a Slaughterhouse in Algeria.Microorganisms · 2024Article
- Article
- Evidence for wastewaters as environments where mobile antibiotic resistance genes emerge.Communications biology · 2023Article
- Structural and Biochemical Features of OXA-517: a Carbapenem and Expanded-Spectrum Cephalosporin Hydrolyzing OXA-48 Variant.Antimicrobial agents and chemotherapy · 2023Article
- Emergence of TnAntimicrobial agents and chemotherapy · 2022Article
- Current Positioning against Severe Infections Due toAntibiotics (Basel, Switzerland) · 2022Review
- OXA-48-Like β-Lactamases: Global Epidemiology, Treatment Options, and Development Pipeline.Antimicrobial agents and chemotherapy · 2022Review
- The biogenesis of β-lactamase enzymes.Microbiology (Reading, England) · 2022Review
- How Do Transposable Elements Activate Expression of Transcriptionally Silent Antibiotic Resistance Genes?International journal of molecular sciences · 2022Review
10 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
IS1999 and a point mutant derivative, IS1999.2, have been described inserted upstream of emerging antibiotic resistance genes bla(VEB-1) and bla(OXA-48). 5' Rapid amplification of cDNA ends experiments revealed that expression of these beta-lactamase genes was driven by the outward-directed promoter, P(out), located in the IS1999 elements. These findings led us to study IS1999-mediated gene mobilization. Thus, the transposition properties of IS1999 and of IS1999-based composite transposons, made of two copies of IS1999 in different orientations, were investigated. IS1999 or IS1999-based composite transposons were capable of transposing onto the conjugative plasmid pOX38-Gen. Sequence analysis of the insertion sites revealed that IS1999 inserted preferentially into DNA targets containing the consensus sequence NGCNNNGCN. Transposition was more efficient when at least one left inverted repeat end was located at an outside end of the transposon. The transposition frequency of IS1999.2 was 10-fold lower than that of IS1999, and transposition frequencies of the putative natural transposon, Tn1999, were below detection limits of our transposition assay. This reduced transposition frequency of IS1999.2-based elements may result from a lower transcription of the transposase gene, as revealed by reverse transcription-PCR analyses.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.