ArticleJournal of bacteriology2006
Tungsten transport protein A (WtpA) in Pyrococcus furiosus: the first member of a new class of tungstate and molybdate transporters.
Article in Journal of bacteriology, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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30 citing papers in PubMed, 78 citations in OpenAlex.
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- The Development of Tungsten Biochemistry-A Personal Recollection.Molecules (Basel, Switzerland) · 2023Review
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- Isolation and Genome Analysis of an Amoeba-Associated BacteriumFrontiers in microbiology · 2022Article
- Tungsten enzymes play a role in detoxifying food and antimicrobial aldehydes in the human gut microbiome.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Characterization of thiosulfate reductase from Pyrobaculum aerophilum heterologously produced in Pyrococcus furiosus.Extremophiles : life under extreme conditions · 2020Article
- Characterization of a Metal-ResistantFrontiers in microbiology · 2020Article
- Determination of Ligand Profiles forInternational journal of molecular sciences · 2019Article
- Article
- Highly selective tungstate transporter protein TupA from Desulfovibrio alaskensis G20.Scientific reports · 2017Article
- Phylogenomics of Rhodobacteraceae reveals evolutionary adaptation to marine and non-marine habitats.The ISME journal · 2017Article
- Transcriptomes of the Extremely Thermoacidophilic Archaeon Metallosphaera sedula Exposed to Metal "Shock" Reveal Generic and Specific Metal Responses.Applied and environmental microbiology · 2016Article
- Metabolic traits of an uncultured archaeal lineage--MSBL1--from brine pools of the Red Sea.Scientific reports · 2016Article
- Biosynthesis and Insertion of the Molybdenum Cofactor.EcoSal Plus · 2015Review
- Acquisition and role of molybdate in Pseudomonas aeruginosa.Applied and environmental microbiology · 2014Article
- TupA: a tungstate binding protein in the periplasm of Desulfovibrio alaskensis G20.International journal of molecular sciences · 2014Article
- Binding of ReO4(-) with an engineered MoO4(2-)-binding protein: towards a new approach in radiopharmaceutical applications.Journal of biological inorganic chemistry : JBIC : a publication of the Society of Biological Inorganic Chemistry · 2012Article
- In silico description of cobalt and nickel assimilation systems in the genomes of methanogens.Systems and synthetic biology · 2011Article
- A molecular basis for tungstate selectivity in prokaryotic ABC transport systems.Journal of bacteriology · 2011Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A novel tungstate and molybdate binding protein has been discovered from the hyperthermophilic archaeon Pyrococcus furiosus. This tungstate transport protein A (WtpA) is part of a new ABC transporter system selective for tungstate and molybdate. WtpA has very low sequence similarity with the earlier-characterized transport proteins ModA for molybdate and TupA for tungstate. Its structural gene is present in the genome of numerous archaea and some bacteria. The identification of this new tungstate and molybdate binding protein clarifies the mechanism of tungstate and molybdate transport in organisms that lack the known uptake systems associated with the ModA and TupA proteins, like many archaea. The periplasmic protein of this ABC transporter, WtpA (PF0080), was cloned and expressed in Escherichia coli. Using isothermal titration calorimetry, WtpA was observed to bind tungstate (dissociation constant [K(D)] of 17 +/- 7 pM) and molybdate (K(D) of 11 +/- 5 nM) with a stoichiometry of 1.0 mol oxoanion per mole of protein. These low K(D) values indicate that WtpA has a higher affinity for tungstate than do ModA and TupA and an affinity for molybdate similar to that of ModA. A displacement titration of molybdate-saturated WtpA with tungstate showed that the tungstate effectively replaced the molybdate in the binding site of the protein.
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