ArticleThe Biochemical journal2007
Pharmacological profiling of the Dictyostelium adenylate cyclases ACA, ACB and ACG.
Article in The Biochemical journal, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
18 citing papers in PubMed, 32 citations in OpenAlex.
- Article
- Diverse Roles of the Multiple Phosphodiesterases in the Regulation of Cyclic Nucleotide Signaling inCells · 2025Review
- Adenylate cyclase A amplification and functional diversification during Polyspondylium pallidum development.EvoDevo · 2022Article
- Caffeine inhibits PI3K and mTORC2 in Dictyostelium and differentially affects multiple other cAMP chemoattractant signaling effectors.Molecular and cellular biochemistry · 2019Article
- Tissue self-organization based on collective cell migration by contact activation of locomotion and chemotaxis.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- Collective cell migration ofCommunications biology · 2019Article
- Evolution of multicellularity in Dictyostelia.The International journal of developmental biology · 2019Review
- Adenylate cyclase A acting on PKA mediates induction of stalk formation by cyclic diguanylate at the Dictyostelium organizer.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- The cyclic AMP phosphodiesterase RegA critically regulates encystation in social and pathogenic amoebas.Cellular signalling · 2014Article
- A RabGAP regulates life-cycle duration via trimeric G-protein cascades in Dictyostelium discoideum.PloS one · 2013Article
- Biological soliton in multicellular movement.Scientific reports · 2013Article
- Regulation of multiple tip formation by caffeine in cellular slime molds.BMC developmental biology · 2012Article
- Regulation of aggregate size and pattern by adenosine and caffeine in cellular slime molds.BMC developmental biology · 2012Article
- Arachidonic acid enhances caffeine-induced cell death via caspase-independent cell death.Scientific reports · 2012Article
- Initiation of Plasmodium sporozoite motility by albumin is associated with induction of intracellular signalling.International journal for parasitology · 2010Article
- Regulatory subunit I-controlled protein kinase A activity is required for apical bile canalicular lumen development in hepatocytes.The Journal of biological chemistry · 2009Article
- Intramolecular activation mechanism of the Dictyostelium LRRK2 homolog Roco protein GbpC.The Journal of biological chemistry · 2008Article
- From drought sensing to developmental control: evolution of cyclic AMP signaling in social amoebas.Molecular biology and evolution · 2008Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Intracellular and secreted cAMPs play crucial roles in controlling cell movement and gene regulation throughout development of the social amoeba Dictyostelium discoideum. cAMP is produced by three structurally distinct ACs (adenylate cyclases), ACA, ACG and ACB, which have distinctive but overlapping patterns of expression and, as concluded from gene disruption studies, seemingly overlapping functions. In addition to gene disruption, acute pharmacological abrogation of protein activity can be a powerful tool to identify the protein's role in the biology of the organism. We analysed the effects of a range of compounds on the activity of ACA, ACB and ACG to identify enzyme-specific modulators. Caffeine, which was previously used to specifically block ACA function, also inhibited cAMP accumulation by ACB and ACG. IPA (2',3'-O-isopropylidene adenosine) specifically inhibits ACA when measured in intact cells, without affecting ACB or ACG. All three enzymes are inhibited by the P-site inhibitor DDA (2',5'-dideoxyadenosine) when assayed in cell lysates, but not in intact cells. Tyrphostin A25 [alpha-cyano-(3,4,5-trihydroxy)cinnamonitrile] and SQ22536 [9-(tetrahydro-2'-furyl)adenine] proved to be effective and specific inhibitors for ACG and ACA respectively. Both compounds acted directly on enzyme activity assayed in cell lysates, but only SQ22536 was also a specific inhibitor when added to intact cells.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.