ArticleNucleic acids research2006
Targeting chromosomal sites with locked nucleic acid-modified triplex-forming oligonucleotides: study of efficiency dependence on DNA nuclear environment.
Article in Nucleic acids research, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 23 citations in OpenAlex.
- Sequence-Specific Targeting of GC-Rich Gene Loci by Parallel Triplex-Forming Oligonucleotides Containing a Modified Nucleobase.bioRxiv : the preprint server for biology · 2026Article
- Preimplantation genetic screening of blastocysts by multiplex qPCR followed by fresh embryo transfer: validation and verification.Molecular cytogenetics · 2015Article
- Locked nucleic acid oligomers as handles for single molecule manipulation.Nucleic acids research · 2014Article
- Dynamic estrogen receptor interactomes control estrogen-responsive trefoil Factor (TFF) locus cell-specific activities.Molecular and cellular biology · 2014Article
- Development of bis-locked nucleic acid (bisLNA) oligonucleotides for efficient invasion of supercoiled duplex DNA.Nucleic acids research · 2013Article
- Homology-directed Fanconi anemia pathway cross-link repair is dependent on DNA replication.Nature structural & molecular biology · 2011Article
- Homologous recombination assay for interstrand cross-link repair.Methods in molecular biology (Clifton, N.J.) · 2011Article
- Nanotechnology approaches for gene transfer.Genetica · 2009Review
- The triple helix: 50 years later, the outcome.Nucleic acids research · 2008Review
- Triplex-forming oligonucleotide-orthophenanthroline conjugates for efficient targeted genome modification.Proceedings of the National Academy of Sciences of the United States of America · 2008Article
- Sequence-specific DNA cleavage mediated by bipyridine polyamide conjugates.Nucleic acids research · 2008Article
- Sequence-specific triple helix formation with genomic DNA.Biochemistry · 2007Article
- Inhibiting gene expression with locked nucleic acids (LNAs) that target chromosomal DNA.Biochemistry · 2007Article
- Bioconjugation of oligonucleotides for treating liver fibrosis.Oligonucleotides · 2007Review
- Cellular antisense activity of peptide nucleic acid (PNAs) targeted to HIV-1 polypurine tract (PPT) containing RNA.Nucleic acids research · 2007Article
Corrections and comments
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Authors and funding
5 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Triplex-forming oligonucleotides (TFOs) are synthetic DNA code-reading molecules that have been demonstrated to function to some extent in chromatin within cell nuclei. Here we have investigated the impact of DNA nuclear environment on the efficiency of TFO binding. For this study we have used locked nucleic acid-containing TFOs (TFO/LNAs) and we report the development of a rapid PCR-based method to quantify triplex formation. We have first compared triplex formation on genes located at different genomic sites and containing the same oligopyrimidine*oligopurine sequence. We have shown that efficient TFO binding is possible on both types of genes, expressed and silent. Then we have further investigated when gene transcription may influence triplex formation in chromatin. We have identified situations where for a given gene, increase of transcriptional activity leads to enhanced TFO binding: this was observed for silent or weakly expressed genes that are not or are only slightly accessible to TFO. Such a transcriptional dependence was observed for integrated and endogenous loci, and chemical and biological activations of transcription. Finally, we provide evidence that TFO binding is sequence-specific as measured on mutated target sequences and that up to 50% of chromosomal targets can be covered by the TFO/LNA in living cells.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.