ArticleApplied and environmental microbiology2006
Quantifying substrate uptake by individual cells of marine bacterioplankton by catalyzed reporter deposition fluorescence in situ hybridization combined with microautoradiography.
Article in Applied and environmental microbiology, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
What it found
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Who cites it
27 citing papers in PubMed, 70 citations in OpenAlex.
- Metabolic inequality in microbial communities.bioRxiv : the preprint server for biology · 2026Article
- Single-cell heterotrophic activity in deep-ocean prokaryotic communities quantified by BONCAT and microautoradiography.ISME communications · 2026Article
- Major contribution of anaplerosis to inorganic carbon fixation in the dark ocean.Nature geoscience · 2026Article
- Metaproteomic analysis decodes trophic interactions of microorganisms in the dark ocean.Nature communications · 2024Article
- Substrate uptake patterns shape niche separation in marine prokaryotic microbiome.Science advances · 2024Article
- A ubiquitous gammaproteobacterial clade dominates expression of sulfur oxidation genes across the mesopelagic ocean.Nature microbiology · 2023Article
- Basin-scale biogeography of Prochlorococcus and SAR11 ecotype replication.The ISME journal · 2023Article
- Nutrient and salt depletion synergistically boosts glucose metabolism in individual Escherichia coli cells.Communications biology · 2022Article
- Limited carbon cycling due to high-pressure effects on the deep-sea microbiome.Nature geoscience · 2022Article
- Using Stable Isotope Probing and Raman Microspectroscopy To Measure Growth Rates of Heterotrophic Bacteria.Applied and environmental microbiology · 2021Article
- Phytoplankton exudates and lysates support distinct microbial consortia with specialized metabolic and ecophysiological traits.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Mesozooplankton taurine production and prokaryotic uptake in the northern Adriatic Sea.Limnology and oceanography · 2020Article
- Bioorthogonal Non-canonical Amino Acid Tagging Combined With Flow Cytometry for Determination of Activity in Aquatic Microorganisms.Frontiers in microbiology · 2020Article
- Visualization is crucial for understanding microbial processes in the ocean.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2019Article
- Taurine Is a Major Carbon and Energy Source for Marine Prokaryotes in the North Atlantic Ocean off the Iberian Peninsula.Microbial ecology · 2019Article
- Single-cell imaging of phosphorus uptake shows that key harmful algae rely on different phosphorus sources for growth.Scientific reports · 2018Article
- Imaging specific newly synthesized proteins within cells by fluorescence resonance energy transfer.Chemical science · 2017Article
- Single-cell activity of freshwater aerobic anoxygenic phototrophic bacteria and their contribution to biomass production.The ISME journal · 2016Article
- Leucine incorporation by aerobic anoxygenic phototrophic bacteria in the Delaware estuary.The ISME journal · 2014Article
- In situ visualization of newly synthesized proteins in environmental microbes using amino acid tagging and click chemistry.Environmental microbiology · 2014Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Catalyzed reporter deposition fluorescence in situ hybridization combined with microautoradiography (MICRO-CARD-FISH) is increasingly being used to obtain qualitative information on substrate uptake by individual members of specific prokaryotic communities. Here we evaluated the potential for using this approach quantitatively by relating the measured silver grain area around cells taking up (3)H-labeled leucine to bulk leucine uptake measurements. The increase in the silver grain area over time around leucine-assimilating cells of coastal bacterial assemblages was linear during 4 to 6 h of incubation. By establishing standardized conditions for specific activity levels and concomitantly performing uptake measurements with the bulk community, MICRO-CARD-FISH can be used quantitatively to determine uptake rates on a single-cell level. Therefore, this approach allows comparisons of single-cell activities for bacterial communities obtained from different sites or growing under different ecological conditions.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.