ArticlePlant physiology2006
Diacidic motif is required for efficient transport of the K+ channel KAT1 to the plasma membrane.
Article in Plant physiology, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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21 citing papers in PubMed, 63 citations in OpenAlex.
- Trafficking and localization of Golgi-residentFrontiers in plant science · 2025Review
- A conserved arginine/lysine-based motif promotes ER export of KCNE1 and KCNE2 to regulate KCNQ1 channel activity.Channels (Austin, Tex.) · 2019Article
- Membrane capacitance recordings resolve dynamics and complexity of receptor-mediated endocytosis in Wnt signalling.Scientific reports · 2019Article
- S-type Anion Channels SLAC1 and SLAH3 Function as Essential Negative Regulators of Inward K+ Channels and Stomatal Opening in Arabidopsis.The Plant cell · 2016Article
- The role of protein-protein interactions in the intracellular traffic of the potassium channels TASK-1 and TASK-3.Pflugers Archiv : European journal of physiology · 2015Review
- Identification of rice cornichon as a possible cargo receptor for the Golgi-localized sodium transporter OsHKT1;3.Journal of experimental botany · 2015Article
- Lost in traffic? The K(+) channel of lily pollen, LilKT1, is detected at the endomembranes inside yeast cells, tobacco leaves, and lily pollen.Frontiers in plant science · 2015Article
- Plant nutrition: root transporters on the move.Plant physiology · 2014Article
- The YXXΦ motif within the severe acute respiratory syndrome coronavirus (SARS-CoV) 3a protein is crucial for its intracellular transport.Virology journal · 2014Article
- Distinct amino acids in the C-linker domain of the Arabidopsis K+ channel KAT2 determine its subcellular localization and activity at the plasma membrane.Plant physiology · 2014Article
- Conduits of life's spark: a perspective on ion channel research since the birth of neuron.Neuron · 2013Review
- The role of K(+) channels in uptake and redistribution of potassium in the model plant Arabidopsis thaliana.Frontiers in plant science · 2013Article
- Article
- The Golgi-localized Arabidopsis endomembrane protein12 contains both endoplasmic reticulum export and Golgi retention signals at its C terminus.The Plant cell · 2012Article
- Salt stress triggers enhanced cycling of Arabidopsis root plasma-membrane aquaporins.Plant signaling & behavior · 2012Article
- Arabidopsis thaliana high-affinity phosphate transporters exhibit multiple levels of posttranslational regulation.The Plant cell · 2011Article
- Preferential KAT1-KAT2 heteromerization determines inward K+ current properties in Arabidopsis guard cells.The Journal of biological chemistry · 2010Article
- The role of the C-terminus for functional heteromerization of the plant channel KDC1.Biophysical journal · 2009Article
- Transport and sorting of the solanum tuberosum sucrose transporter SUT1 is affected by posttranslational modification.The Plant cell · 2008Article
- ER membrane aquaporins in plants.Pflugers Archiv : European journal of physiology · 2008Review
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
For a number of mammalian ion channels, trafficking to the plasma membrane was found to be controlled by intrinsic sequence motifs. Among these sequences are diacidic motifs that function as endoplasmic reticulum (ER) export signals. So far it is unclear if similar motifs also exist in plant ion channels. In this study we analyzed the function of four diacidic DXE/DXD motifs of the plant K(+) channel KAT1. Mutation of the first diacidic DXE motif resulted in a strong reduction of the KAT1 conductance in both guard cell protoplasts and HEK293 cells (human embryonic kidney cells). Confocal fluorescence microscopy of guard cells expressing the mutated KAT1 fused to green fluorescent protein revealed localization of the mutated channel only in intracellular structures around the nucleus. These structures could be identified as part of the ER via coexpression of KAT1 fused to yellow fluorescent protein with an ER-retained protein (HDEL) fused to cyan fluorescent protein. Block of vesicle formation from the ER by overexpression of the small GTP-binding protein Sar1 fixed in its GDP-bound form led to retention of wild-type KAT1 in similar parts of the ER. Mutation of the three other diacidic motifs had no effect. Together, the results demonstrate that one diacidic motif of KAT1 is essential for ER export of the functional channel in both guard cell protoplasts and HEK293 cells. This suggests that trafficking of plant plasma membrane ion channels is controlled via a conserved mechanism.
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