ArticleBiophysical journal2006
The role of F-actin and myosin in epithelial cell rheology.
Article in Biophysical journal, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 42 papers.
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Who cites it
42 citing papers in PubMed, 124 citations in OpenAlex.
- Cellular and Nuclear Forces: An Overview.Methods in molecular biology (Clifton, N.J.) · 2025Review
- Myosin-independent stiffness sensing by fibroblasts is regulated by the viscoelasticity of flowing actin.Communications materials · 2024Article
- Evidence that tissue recoil in the earlyMolecular biology of the cell · 2023Article
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- Imaging methods in mechanosensing: a historical perspective and visions for the future.Molecular biology of the cell · 2021Review
- 3D Cell Culture for the Study of Microenvironment-Mediated Mechanostimuli to the Cell Nucleus: An Important Step for Cancer Research.Frontiers in molecular biosciences · 2021Article
- Dynamic actin cross-linking governs the cytoplasm's transition to fluid-like behavior.Molecular biology of the cell · 2020Article
- Dynamic heterogeneity and non-Gaussian statistics for ganglioside GM1s and acetylcholine receptors on live cell membrane.Molecular biology of the cell · 2020Article
- Filament Rigidity Vies with Mesh Size in Determining Anomalous Diffusion in Cytoskeleton.Biomacromolecules · 2019Article
- Dissecting fat-tailed fluctuations in the cytoskeleton with active micropost arrays.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- Blood pressure-induced physiological strain variability modulates wall structure and function in aorta rings.Physiological measurement · 2018Article
- Prestressed cells are prone to cytoskeleton failures under localized shear strain: an experimental demonstration on muscle precursor cells.Scientific reports · 2018Article
- Temporal Variation in Single-Cell Power-Law Rheology Spans the Ensemble Variation of Cell Population.Biophysical journal · 2017Article
- A microfluidic chamber-based approach to map the shear moduli of vascular cells and other soft materials.Scientific reports · 2017Article
- Integrated Analysis of Intracellular Dynamics of MenaINV Cancer Cells in a 3D Matrix.Biophysical journal · 2017Article
- Mapping intracellular mechanics on micropatterned substrates.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Mechanical properties of normal versus cancerous breast cells.Biomechanics and modeling in mechanobiology · 2015Article
- Human Primary Immune Cells Exhibit Distinct Mechanical Properties that Are Modified by Inflammation.Biophysical journal · 2015Article
- The mechanical properties of early Drosophila embryos measured by high-speed video microrheology.Biophysical journal · 2015Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Although actin and myosin are important contributors to cell-force generation, shape change, and motility, their contributions to cell stiffness and frequency-dependent rheology have not been conclusively determined. We apply several pharmacological interventions to cultured epithelial cells to elucidate the roles of actin and myosin in the mechanical response of cells and intracellular fluctuations. A suite of different methods is used to separately examine the mechanics of the deep cell interior and cortex, in response to depletion of intracellular ATP, depolymerization of F-actin, and inhibition of myosin II. Comparison of these results shows that F-actin plays a significant role in the mechanics of the cortical region of epithelial cells, but its disruption has no discernable effect on the rheology of the deeper interior. Moreover, we find that myosins do not contribute significantly to the rheology or ATP-dependent, non-Brownian motion in the cell interior. Finally, we investigate the broad distribution of apparent stiffness values reported by some microrheology methods, which are not observed with two-point microrheology. Based on our findings and a simple model, we conclude that heterogeneity of the tracer-cytoskeleton contacts, rather than the network itself, can explain the broad distribution of apparent stiffnesses.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.