ArticleBiophysical journal2006
Spatially resolved fluorescence correlation spectroscopy using a spinning disk confocal microscope.
Article in Biophysical journal, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 69 citations in OpenAlex.
- Super-resolution algorithms for imaging FCS enhancement: A comparative study.Biophysical journal · 2025Article
- Segmented fluorescence correlation spectroscopy (FCS) on a commercial laser scanning microscope.Scientific reports · 2024Article
- Current capabilities and future perspectives of FCS: super-resolution microscopy, machine learning, and in vivo applications.Communications biology · 2023Review
- Confocal-based fluorescence fluctuation spectroscopy with a SPAD array detector.Light, science & applications · 2021Article
- Using enhanced number and brightness to measure protein oligomerization dynamics in live cells.Nature protocols · 2019Article
- Visualization of barriers and obstacles to molecular diffusion in live cells by spatial pair-cross-correlation in two dimensions.Biomedical optics express · 2018Article
- Local raster image correlation spectroscopy generates high-resolution intracellular diffusion maps.Communications biology · 2018Article
- Imaging fluorescence (cross-) correlation spectroscopy in live cells and organisms.Nature protocols · 2015Article
- Exploring the dynamics of cell processes through simulations of fluorescence microscopy experiments.Biophysical journal · 2015Article
- Bayesian total internal reflection fluorescence correlation spectroscopy reveals hIAPP-induced plasma membrane domain organization in live cells.Biophysical journal · 2014Article
- Fibrin networks regulate protein transport during thrombus development.PLoS computational biology · 2013Article
- Cellular response to heat shock studied by multiconfocal fluorescence correlation spectroscopy.Biophysical journal · 2012Article
- Monitoring dynamic binding of chromatin proteins in vivo by fluorescence correlation spectroscopy and temporal image correlation spectroscopy.Methods in molecular biology (Clifton, N.J.) · 2012Article
- Dissecting chromatin interactions in living cells from protein mobility maps.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2011Article
- High-throughput FCS using an LCOS spatial light modulator and an 8 × 1 SPAD array.Biomedical optics express · 2010Article
- Cross-validating FRAP and FCS to quantify the impact of photobleaching on in vivo binding estimates.Biophysical journal · 2010Article
- A generative model of microtubule distributions, and indirect estimation of its parameters from fluorescence microscopy images.Cytometry. Part A : the journal of the International Society for Analytical Cytology · 2010Article
- Three-dimensional Flow Mapping in Microfiuidic Channels with Widefield Cross-correlation Microscopy.Israel journal of chemistry · 2010Article
- Recent developments in fluorescence correlation spectroscopy for diffusion measurements in planar lipid membranes.International journal of molecular sciences · 2010Review
- Image correlation microscopy for uniform illumination.Journal of microscopy · 2010Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
We develop an extension of fluorescence correlation spectroscopy (FCS) using a spinning disk confocal microscope. This approach can spatially map diffusion coefficients or flow velocities at up to approximately 10(5) independent locations simultaneously. Commercially available cameras with frame rates of 1000 Hz allow FCS measurements of systems with diffusion coefficients D~10(-7) cm(2)/s or smaller. This speed is adequate to measure small microspheres (200-nm diameter) diffusing in water, or hindered diffusion of macromolecules in complex media (e.g., tumors, cell nuclei, or the extracellular matrix). There have been a number of recent extensions to FCS based on laser scanning microscopy. Spinning disk confocal microscopy, however, has the potential for significantly higher speed at high spatial resolution. We show how to account for a pixel size effect encountered with spinning disk confocal FCS that is not present in standard or scanning FCS, and we introduce a new method to correct for photobleaching. Finally, we apply spinning disk confocal FCS to microspheres diffusing in Type I collagen, which show complex spatially varying diffusion caused by hydrodynamic and steric interactions with the collagen matrix.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.