ArticleBMC genomics2006
Development and validation of a bovine macrophage specific cDNA microarray.
Article in BMC genomics, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 43 citations in OpenAlex.
- Highly multiplexed quantitative PCR-based platform for evaluation of chicken immune responses.PloS one · 2019Article
- Microarray analysis of differential gene expression profiles in blood cells of naturally BLV-infected and uninfected Holstein-Friesian cows.Molecular biology reports · 2017Article
- Novel identification of Factor XI deficiency in Indian Sahiwal (Bos indicus) cattle.Molecular biology reports · 2016Article
- Phenotypic and functional analysis of monocyte populations in cattle peripheral blood identifies a subset with high endocytic and allogeneic T-cell stimulatory capacity.Veterinary research · 2015Article
- The mRNA expression of immune-related genes in crossbred and Tharparkar cattle in response to in vitro infection with Theileria annulata.Molecular biology reports · 2015Article
- Functional analysis of bovine TLR5 and association with IgA responses of cattle following systemic immunisation with H7 flagella.Veterinary research · 2015Article
- Comparison of small interfering RNA (siRNA) delivery into bovine monocyte-derived macrophages by transfection and electroporation.Veterinary immunology and immunopathology · 2014Article
- The level of H₂O₂ type oxidative stress regulates virulence of Theileria-transformed leukocytes.Cellular microbiology · 2014Article
- The early phase transcriptome of bovine monocyte-derived macrophages infected with Staphylococcus aureus in vitro.BMC genomics · 2013Article
- Differential response of bovine mammary epithelial cells to Staphylococcus aureus or Escherichia coli agonists of the innate immune system.Veterinary research · 2013Article
- Escherichia coli- and Staphylococcus aureus-induced mastitis differentially modulate transcriptional responses in neighbouring uninfected bovine mammary gland quarters.BMC genomics · 2013Article
- Article
- Neuroimmunoendocrine regulation of the prion protein in neutrophils.The Journal of biological chemistry · 2012Article
- Living with the enemy or uninvited guests: functional genomics approaches to investigating host resistance or tolerance traits to a protozoan parasite, Theileria annulata, in cattle.Veterinary immunology and immunopathology · 2012Review
- Article
- The protozoan parasite Theileria annulata alters the differentiation state of the infected macrophage and suppresses musculoaponeurotic fibrosarcoma oncogene (MAF) transcription factors.International journal for parasitology · 2009Article
- Development and validation of an oligonucleotide microarray for immuno-inflammatory genes of ruminants.Veterinary research communications · 2008Article
- Design and evaluation of Actichip, a thematic microarray for the study of the actin cytoskeleton.BMC genomics · 2007Article
- Directional responses following recombinant cytokine stimulation of rainbow trout (Oncorhynchus mykiss) RTS-11 macrophage cells as revealed by transcriptome profiling.BMC genomics · 2007Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe response of macrophages to danger signals is an important early stage in the immune response. Our understanding of this complex event has been furthered by microarray analysis, which allows the simultaneous investigation of the expression of large numbers of genes. However, the microarray resources available to study these events in livestock animals are limited.
resultsHere we report the development of a bovine macrophage specific (BoMP) cDNA microarray. The BoMP microarray contains 5026 sequence elements (printed in duplicate) and numerous controls. The majority of the clones incorporated on the microarray were derived from the BoMP cDNA library generated from bovine myeloid cells subjected to various stimuli, including over 900 sequences unique to the library. Additional clones representing immunologically important genes have been included on the BoMP microarray. The microarray was validated by investigating the response of bovine monocytes to stimulation with interferon-gamma and lipopolysaccharide using amplified RNA. At 2 and 16 hours post stimulation 695 genes exhibited statistically significant differential expression, including; 26 sequences unique to the BoMP library, interleukin 6, prion protein and toll-like receptor 4.
conclusionA 5 K cDNA microarray has been successfully developed to investigate gene expression in bovine myeloid cells. The BoMP microarray is available from the ARK-Genomics Centre for Functional Genomics in Farm Animals, UK.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.