ArticleIn vitro cellular & developmental biology. Animal
Strategy for the development of a matched set of transport-competent, angiotensin receptor-deficient proximal tubule cell lines.
Article in In vitro cellular & developmental biology. Animal. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 22 citations in OpenAlex.
- Insights into Repeated Renal Injury Using RNA-Seq with Two New RPTEC Cell Lines.International journal of molecular sciences · 2023Article
- Intracellular Angiotensin II Stimulation of Sodium Transporter Expression in Proximal Tubule Cells via ATCells · 2023Article
- Selenium deficiency causes hypertension by increasing renal ATFree radical biology & medicine · 2023Article
- Gastrin, via activation of PPARα, protects the kidney against hypertensive injury.Clinical science (London, England : 1979) · 2021Article
- Evidence for a Physiological Mitochondrial Angiotensin II System in the Kidney Proximal Tubules: Novel Roles of Mitochondrial Ang II/ATHypertension (Dallas, Tex. : 1979) · 2020Article
- Renal Hydrogen Peroxide Production Prevents Salt-Sensitive Hypertension.Journal of the American Heart Association · 2020Article
- Dopamine DScientific reports · 2019Article
- Augmentation of angiotensinogen expression in the proximal tubule by intracellular angiotensin II via AT1a/MAPK/NF-кB signaling pathways.American journal of physiology. Renal physiology · 2016Article
- Dopamine D2 receptors' effects on renal inflammation are mediated by regulation of PP2A function.American journal of physiology. Renal physiology · 2016Article
- Role of caveolin 1 in AT1a receptor-mediated uptake of angiotensin II in the proximal tubule of the kidney.American journal of physiology. Renal physiology · 2014Article
- Mechanisms of AT1a receptor-mediated uptake of angiotensin II by proximal tubule cells: a novel role of the multiligand endocytic receptor megalin.American journal of physiology. Renal physiology · 2014Article
- Proximal tubule-dominant transfer of AT(1a) receptors induces blood pressure responses to intracellular angiotensin II in AT(1a) receptor-deficient mice.American journal of physiology. Regulatory, integrative and comparative physiology · 2013Article
- Novel signaling mechanisms of intracellular angiotensin II-induced NHE3 expression and activation in mouse proximal tubule cells.American journal of physiology. Renal physiology · 2012Article
- Role of renal DJ-1 in the pathogenesis of hypertension associated with increased reactive oxygen species production.Hypertension (Dallas, Tex. : 1979) · 2012Article
- Deficient dopamine D2 receptor function causes renal inflammation independently of high blood pressure.PloS one · 2012Article
- A novel approach for the generation of genetically modified mammary epithelial cell cultures yields new insights into TGFβ signaling in the mammary gland.Breast cancer research : BCR · 2010Article
- AT1 receptor-mediated uptake of angiotensin II and NHE-3 expression in proximal tubule cells through a microtubule-dependent endocytic pathway.American journal of physiology. Renal physiology · 2009Article
- Beta-arrestin-mediated localization of smoothened to the primary cilium.Science (New York, N.Y.) · 2008Article
- Tissue and subcellular distribution of CLIC1.BMC cell biology · 2007Article
- Development of an AT2-deficient proximal tubule cell line for transport studies.In vitro cellular & developmental biology. AnimalArticle
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Authors and funding
7 authors at 5 institutions in 3 countries.
Funding
Abstract
In the proximal convoluted tubule (PCT) angiotensin II (Ang II) modulates fluid and electrolyte transport through at least two pharmacologically distinct receptor subtypes: AT(1) and AT(2). Development of cell lines that lack these receptors are potentially useful models to probe the complex cellular details of Ang II regulation. To this end, angiotensin receptor- deficient mice were bred with an Immortomouse(R), which harbors a thermolabile SV40 large-T antigen (Tag). S1 PCT segments from kidneys of F(2) mice were microdissected, placed in culture, and maintained under conditions that enhanced cell growth, i.e., promoted Tag expression and thermostability. Three different types of angiotensin receptor-deficient cell lines, (AT(1A) [-/-], Tag [+/-]), (AT(1B) [-/-], Tag [+/-]), and (AT(1A) [-/-], AT(1B) [-/-], Tag [+/+]), as well as wild type cell lines were generated. Screening and characterization, which were conducted under culture conditions that promoted cellular differentiation, included: measurements of transepithelial transport, such as basal monolayer short-circuit current (Isc; -3 to 3 microA/cm2), basal monolayer conductance (G, 2 to 10 mS/cm2), Na3(+)-phosphate cotransport (DeltaIsc of 2 to 3 microA/cm(2) at 1 mM), and Na(3)(+)-succinate cotransport (DeltaIsc of 1 to 9 microA/cm(2) at 2 mM). Morphology of cell monolayers showed an extensive brush border, well-defined tight junctions, and primary cilia. Receptor functionality was assessed by Ang II-stimulated beta-arrestin 2 translocation and showed an Ang II-mediated response in wild type but not (AT(1A) [-/ -], AT(1B) [-/-]) cells. Cell lines were amplified, yielding a virtually unlimited supply of highly differentiated, transport-competent, angiotensin receptor-deficient PCT cell lines.
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