ArticleThe EMBO journal2006
Mechanism of RecA-mediated homologous recombination revisited by single molecule nanomanipulation.
Article in The EMBO journal, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
24 citing papers in PubMed, 43 citations in OpenAlex.
- Chromosome topology gates productive RecA homology search.bioRxiv : the preprint server for biology · 2026Article
- NProceedings of the National Academy of Sciences of the United States of America · 2025Article
- During recombinase-mediated homology recognition RecQ helicases inhibit formation of toxic long-lived D-loops that could promote genomic instability.Nucleic acids research · 2025Article
- Single-molecule insight into stalled replication fork rescue in Escherichia coli.Nucleic acids research · 2021Review
- Mismatch detection in homologous strand exchange amplified by hydrophobic effects.Biopolymers · 2021Article
- The ATPase activity of E. coli RecA prevents accumulation of toxic complexes formed by erroneous binding to undamaged double stranded DNA.Nucleic acids research · 2018Article
- Dissection of structural dynamics of chromatin fibers by single-molecule magnetic tweezers.Biophysics reports · 2018Article
- Multi-scale tracking reveals scale-dependent chromatin dynamics after DNA damage.Molecular biology of the cell · 2017Article
- On the Mechanism of Homology Search by RecA Protein Filaments.Biophysical journal · 2017Article
- An Overview of the Molecular Mechanisms of Recombinational DNA Repair.Cold Spring Harbor perspectives in biology · 2015Review
- Mechanisms and principles of homology search during recombination.Nature reviews. Molecular cell biology · 2014Review
- Complementary strand relocation may play vital roles in RecA-based homology recognition.Nucleic acids research · 2012Article
- Single-molecule imaging of DNA pairing by RecA reveals a three-dimensional homology search.Nature · 2012Article
- Single-molecule views of protein movement on single-stranded DNA.Annual review of biophysics · 2012Review
- Real-time observation of strand exchange reaction with high spatiotemporal resolution.Structure (London, England : 1993) · 2011Article
- Developing single-molecule TPM experiments for direct observation of successful RecA-mediated strand exchange reaction.PloS one · 2011Article
- Single-molecule imaging brings Rad51 nucleoprotein filaments into focus.Trends in cell biology · 2010Review
- Visualizing protein-DNA interactions at the single-molecule level.Current opinion in chemical biology · 2010Review
- Direct imaging of human Rad51 nucleoprotein dynamics on individual DNA molecules.Proceedings of the National Academy of Sciences of the United States of America · 2009Article
- Single molecule studies of homologous recombination.Molecular bioSystems · 2008Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The mechanisms of RecA-mediated three-strand homologous recombination are investigated at the single-molecule level, using magnetic tweezers. Probing the mechanical response of DNA molecules and nucleoprotein filaments in tension and in torsion allows a monitoring of the progression of the exchange in real time, both from the point of view of the RecA-bound single-stranded DNA and from that of the naked double-stranded DNA (dsDNA). We show that strand exchange is able to generate torsion even along a molecule with freely rotating ends. RecA readily depolymerizes during the reaction, a process presenting numerous advantages for the cell's 'protein economy' and for the management of topological constraints. Invasion of an untwisted dsDNA by a nucleoprotein filament leads to an exchanged duplex that remains topologically linked to the exchanged single strand, suggesting multiple initiations of strand exchange on the same molecule. Overall, our results seem to support several important assumptions of the monomer redistribution model.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.