ArticleProceedings of the National Academy of Sciences of the United States of America2006
Phosphorylation of actin Tyr-53 inhibits filament nucleation and elongation and destabilizes filaments.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.
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Who cites it
38 citing papers in PubMed, 65 citations in OpenAlex.
- Effect of the combined action of myofibrillar protein phosphorylation and S-nitrosylation on the degradation of myofibrillar proteins by µ-calpain.Food science of animal resources · 2026Article
- The O-GlcNAcylation of β-actin Ser199 controls nuclear speckle localization and is dysregulated in diabetes.Histochemistry and cell biology · 2025Article
- Alpha-dystroglycan receptor signaling likely influences basement membrane laminin α2 mediated pathology in the stria vascularis of Alport mice.Hearing research · 2025Article
- Multiple Mechanisms to Regulate Actin Functions: "Fundamental" Versus Lineage-Specific Mechanisms and Hierarchical Relationships.Biomolecules · 2025Review
- Aggravated Ulcerative Colitis via circNlgn-Mediated Suppression of Nuclear Actin Polymerization.Research (Washington, D.C.) · 2024Article
- From amino-acid to disease: the effects of oxidation on actin-myosin interactions in muscle.Journal of muscle research and cell motility · 2023Review
- Omics Analyses ofGenes · 2022Article
- Posttranslational modifications of the cytoskeleton.Cytoskeleton (Hoboken, N.J.) · 2021Review
- Targeting cytoskeletal phosphorylation in cancer.Exploration of targeted anti-tumor therapy · 2021Review
- Lysine acetylation of cytoskeletal proteins: Emergence of an actin code.The Journal of cell biology · 2020Review
- Actin R256 Mono-methylation Is a Conserved Post-translational Modification Involved in Transcription.Cell reports · 2020Article
- Age-Onset Phosphorylation of a Minor Actin Variant Promotes Intestinal Barrier Dysfunction.Developmental cell · 2019Article
- Article
- Oxidative hotspots on actin promote skeletal muscle weakness in rheumatoid arthritis.JCI insight · 2019Article
- Using the SpyTag SpyCatcher system to label smooth muscle myosin II filaments with a quantum dot on the regulatory light chain.Cytoskeleton (Hoboken, N.J.) · 2019Article
- ANGUSTIFOLIA Regulates Actin Filament Alignment for Nuclear Positioning in Leaves.Plant physiology · 2019Article
- Microtubule-Based Control of Motor-Clutch System Mechanics in Glioma Cell Migration.Cell reports · 2018Article
- Regulated methionine oxidation by monooxygenases.Free radical biology & medicine · 2017Review
- STK16 regulates actin dynamics to control Golgi organization and cell cycle.Scientific reports · 2017Article
- Review
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
Dictyostelium actin was shown to become phosphorylated on Tyr-53 late in the developmental cycle and when cells in the amoeboid stage are subjected to stress but the phosphorylated actin had not been purified and characterized. We have separated phosphorylated and unphosphorylated actin and shown that Tyr-53 phosphorylation substantially reduces actin's ability to inactivate DNase I, increases actin's critical concentration, and greatly reduces its rate of polymerization. Tyr-53 phosphorylation substantially, if not completely, inhibits nucleation and elongation from the pointed end of actin filaments and reduces the rate of elongation from the barbed end. Negatively stained electron microscopic images of polymerized Tyr-53-phosphorylated actin show a variable mixture of small oligomers and filaments, which are converted to more typical, long filaments upon addition of myosin subfragment 1. Tyr-53-phosphorylated and unphosphorylated actin copolymerize in vitro, and phosphorylated and unphosphorylated actin colocalize in amoebae. Tyr-53 phosphorylation does not affect the ability of filamentous actin to activate myosin ATPase.
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