ArticleLipids in health and disease2006
Syndecan-1 mediates internalization of apoE-VLDL through a low density lipoprotein receptor-related protein (LRP)-independent, non-clathrin-mediated pathway.
Article in Lipids in health and disease, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 57 citations in OpenAlex.
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- Calcium dobesilate reduces SARS-CoV-2 entry into endothelial cells by inhibiting virus binding to heparan sulfate.Scientific reports · 2022Article
- The Interplay of Apoes with Syndecans in Influencing Key Cellular Events of Amyloid Pathology.International journal of molecular sciences · 2021Article
- Pro-metastatic functions of lipoproteins and extracellular vesicles in the acidic tumor microenvironment.Cancer metastasis reviews · 2019Review
- Identification of key genes and pathways in uterine leiomyosarcoma through bioinformatics analysis.Oncology letters · 2018Article
- Hepatic and Aortic Arch Expression and Serum Levels of Syndecan-1 in ApoEThe open biochemistry journal · 2017Article
- HS3ST2 expression is critical for the abnormal phosphorylation of tau in Alzheimer's disease-related tau pathology.Brain : a journal of neurology · 2015Article
- Apolipoprotein E in synaptic plasticity and Alzheimer's disease: potential cellular and molecular mechanisms.Molecules and cells · 2014Review
- Syndecan-1 is required to maintain intradermal fat and prevent cold stress.PLoS genetics · 2014Article
- The productive entry pathway of HIV-1 in macrophages is dependent on endocytosis through lipid rafts containing CD4.PloS one · 2014Article
- Clathrin-independent endocytosis: a cargo-centric view.Experimental cell research · 2013Review
- 6-o- and N-sulfated syndecan-1 promotes baculovirus binding and entry into Mammalian cells.Journal of virology · 2013Article
- Apolipoproteins E and AV mediate lipoprotein clearance by hepatic proteoglycans.The Journal of clinical investigation · 2013Article
- LDL receptor and its family members serve as the cellular receptors for vesicular stomatitis virus.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- Shedding of syndecan-1 from human hepatocytes alters very low density lipoprotein clearance.Hepatology (Baltimore, Md.) · 2012Article
- Syndecan-1 antigen, a promising new target for triple-negative breast cancer immuno-PET and radioimmunotherapy. A preclinical study on MDA-MB-468 xenograft tumors.EJNMMI research · 2011Article
- Guanidinylated neomycin mediates heparan sulfate-dependent transport of active enzymes to lysosomes.Molecular therapy : the journal of the American Society of Gene Therapy · 2010Article
- Role of syndecan-3 polymorphisms in obesity and female hyperandrogenism.Journal of molecular medicine (Berlin, Germany) · 2009Article
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3 authors at 1 institution in 1 country.
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Abstract
backgroundTriacylglyerol-rich very low density lipoprotein (VLDL) particles are the primary carriers of fatty acids in the circulation and as such serve as a rich energy source for peripheral tissues. Receptor-mediated uptake of these particles is dependent upon prior association with apolipoprotein E (apoE-VLDL) and is brought about by cell surface heparan sulfate proteoglycans (HSPG) in some cell types and by the low density lipoprotein receptor-related protein (LRP) in others. Although LRP's role in apoE-VLDL uptake has been well studied, the identity of the HSPG family member that mediates apoE-VLDL uptake has not been established. We investigated if syndecan-1 (Syn-1), a transmembrane cell surface HSPG, is able to mediate the internalization of apoE-VLDL and examined the relationship between Syn-1 and LRP toward apoE-VLDL uptake. For this study, we used a human fibroblast cell line (GM00701) that expresses large amounts of LRP, but possesses no LDL receptor activity to eliminate its contributions toward apoE-VLDL uptake.
resultsAlthough LRP in these cells is fully active as established by substantial alpha2macroglobulin binding and internalization, uptake of apoE-VLDL is absent. Expression of human Syn-1 cDNA restored apoE-VLDL binding and uptake by these cells. Competition for this uptake with an LRP ligand-binding antagonist had little or no effect, whereas co-incubation with heparin abolished apoE-VLDL internalization. Depleting Syn-1 expressing cells of K+, to block clathrin-mediated endocytosis, showed no inhibition of Syn-1 internalization of apoE-VLDL. By contrast, treatment of cells with nystatin to inhibit lipid raft function, prevented the uptake of apoE-VLDL by Syn-1.
conclusionThese data demonstrate that Syn-1 is able to mediate apoE-VLDL uptake in human fibroblasts with little or no contribution from LRP and that the endocytic path taken by Syn-1 is clathrin-independent and relies upon lipid raft function. These data are consistent with previous studies demonstrating Syn-1 association with lipid raft domains.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.