ArticleJournal of clinical microbiology2006
Multiplex PCR-based method for identification of common clinical serotypes of Salmonella enterica subsp. enterica.
Article in Journal of clinical microbiology, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 51 papers.
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Who cites it
51 citing papers in PubMed.
- Characterization and evaluation of a phage cocktail targeting Salmonella enterica in a Turkey farm.BMC microbiology · 2026Article
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- Prevalence, Antibiotic Resistance, and Biofilm Formation of Proteus mirabilis in Dairy Products: Implications for Veterinary and Public Health.Veterinary medicine and science · 2025Article
- Resistance, virulence and genetic diversity of Salmonella Typhimurium in South Africa (1999-2021).The Onderstepoort journal of veterinary research · 2025Article
- Serotyping and Antibiotic Resistance Profiles ofVeterinary sciences · 2025Article
- A Novel LyticFoods (Basel, Switzerland) · 2025Article
- Emergence and Clonal Spread of Extended-Spectrum β-Lactamase-ProducingAntibiotics (Basel, Switzerland) · 2025Article
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- Genomic and Transcriptomic Comparison Between Invasive Non-TyphoidalMicroorganisms · 2024Article
- Characterization of Salmonella species from poultry slaughterhouses in South Korea: carry-over transmission ofJournal of veterinary science · 2024Article
- Prevalence Estimation, Antimicrobial Susceptibility, and Serotyping ofAntibiotics (Basel, Switzerland) · 2023Article
- Assessment of the prevalence, serotype, and antibiotic resistance pattern ofFrontiers in microbiology · 2023Article
- The Occurrence and Genomic Characteristics ofFoods (Basel, Switzerland) · 2022Article
- Prevalence, Serotypes, and Antimicrobial Resistance Patterns of Non-TyphoidPathogens (Basel, Switzerland) · 2022Article
- Emergence and spread in Taiwan of multidrug-resistant serotypes of nontyphoidal Salmonella.Infection · 2022Article
- Virulence and antimicrobial resistance factors inFrontiers in veterinary science · 2022Article
- Salmonella Genomics in Public Health and Food Safety.EcoSal Plus · 2021Article
- Antimicrobial Resistance Profiles ofPathogens (Basel, Switzerland) · 2021Article
- Determinants of Childhood Zoonotic Enteric Infections in a Semirural Community of Quito, Ecuador.The American journal of tropical medicine and hygiene · 2020Article
- Article
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A multiplex PCR method has been developed to differentiate between the most common clinical serotypes of Salmonella enterica subsp. enterica encountered in Washington State and the United States in general. Six genetic loci from S. enterica serovar Typhimurium and four from S. enterica serovar Typhi were used to create an assay consisting of two five-plex PCRs. The assays gave reproducible results with 30 different serotypes that represent the most common clinical isolates of S. enterica subsp. enterica. Of these, 22 serotypes gave unique amplification patterns compared with each other and the other 8 serotypes were grouped into four pairs. These were further resolved by two additional PCRs. We compared the data from PCR serotyping with conventional serotyping and found that PCR serotyping was nearly as discriminatory as conventional serotyping was. The results from a blind test screening 111 clinical isolates revealed that 97% were correctly identified using the multiplex PCR assay. The assay can be easily performed on multiple samples with final results in less than 5 h and, in conjunction with pulsed-field gel electrophoresis, forms a very robust test method for the molecular subtyping of Salmonella enterica subsp. enterica.
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