ArticleThe British journal of ophthalmology2007
Selective blockade of phosphodiesterase types 2, 5 and 9 results in cyclic 3'5' guanosine monophosphate accumulation in retinal pigment epithelium cells.
Article in The British journal of ophthalmology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 27 citations in OpenAlex.
- Choroidal and retinal alteration after long-term use of tadalafil: a prospective non-randomized clinical trial.Journal of basic and clinical physiology and pharmacology · 2024Trial
- Article
- The sleep quality- and myopia-linked PDE11A-Y727C variant impacts neural physiology by reducing catalytic activity and altering subcellular compartmentalization of the enzyme.bioRxiv : the preprint server for biology · 2023Article
- Retinal and Optic Disc Vascular Changes in Patients Using Long-Term Tadalafil: A Prospective Non-Randomized Matched-Pair Study.Diagnostics (Basel, Switzerland) · 2021Article
- Hydrogen sulfide: a gaseous signaling molecule modulates tissue homeostasis: implications in ophthalmic diseases.Cell death & disease · 2019Review
- The Phosphodiesterase 9 Inhibitor PF-04449613 Promotes Dendritic Spine Formation and Performance Improvement after Motor Learning.Developmental neurobiology · 2018Article
- Phosphodiesterase 9 (PDE9) regulates bovine tracheal smooth muscle relaxation.The Journal of veterinary medical science · 2018Article
- Pathophysiology of visual disorders induced by phosphodiesterase inhibitors in the treatment of erectile dysfunction.Drug design, development and therapy · 2016Review
- PDE9A is expressed in the inner retina and contributes to the normal shape of the photopic ERG waveform.Frontiers in molecular neuroscience · 2014Article
- Inhibition of phosphodiestrase 9 induces cGMP accumulation and apoptosis in human breast cancer cell lines, MCF-7 and MDA-MB-468.Cell proliferation · 2012Article
- cGMP-dependent protein kinases and cGMP phosphodiesterases in nitric oxide and cGMP action.Pharmacological reviews · 2010Review
- Insight into binding of phosphodiesterase-9A selective inhibitors by crystal structures and mutagenesis.Journal of medicinal chemistry · 2010Article
- Immunohistochemical localization of phosphodiesterase 2A in multiple mammalian species.The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society · 2009Article
- Cyclic GMP in the pig vitreous and retina after experimental retinal detachment.Molecular vision · 2008Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
aimTo investigate which phosphodiesterase (PDE) is involved in regulating cyclic 3'5' guanosine monophosphate breakdown in retinal pigment epithelium (RPE) cells.
methodscGMP content in the cultured RPE cells (D407 cell line) was evaluated by immunocytochemistry in the presence of non-selective or isoform-selective PDE inhibitors in combination with the particulate guanylyl cyclase stimulator atrial natriuretic peptide (ANP) or the soluble guanylyl cyclase stimulator sodium nitroprusside (SNP). mRNA expression of PDE2, PDE5 and PDE9 was studied in cultured human RPE cells and rat RPE cell layers using non-radioactive in situ hybridisation.
resultsIn the absence of PDE inhibitors, cGMP levels in cultured RPE cells are very low. cGMP accumulation was readily detected in cultured human RPE cells after incubation with Bay60-7550 as a selective PDE2 inhibitor, sildenafil as a selective PDE5 inhibitor or Sch51866 as a selective PDE9 inhibitor. In the presence of PDE inhibition, cGMP content increased markedly after stimulation of the particulate guanylyl cyclase. mRNA of PDE2,PDE5 and PDE9 was detected in all cultured human RPE cells and also in rat RPE cell layers.
conclusionsPDE2, PDE5 and PDE9 have a role in cGMP metabolism in RPE cells.
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