ArticleIn vitro cellular & developmental biology : journal of the Tissue Culture Association1990
Human oral epithelial cell culture. II. Keratin expression in fetal and adult gingival cells.
Article in In vitro cellular & developmental biology : journal of the Tissue Culture Association, 1990. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 39 citations in OpenAlex.
- Illuminating the oral microbiome: cellular microbiology.FEMS microbiology reviews · 2023Article
- Protein S drives oral squamous cell carcinoma tumorigenicity through regulation of AXL.Oncotarget · 2017Article
- Fetal stem cell transplantation: Past, present, and future.World journal of stem cells · 2014Review
- Bacterial invasion of epithelial cells and spreading in periodontal tissue.Periodontology 2000 · 2010Review
- Infection of human cytomegalovirus in cultured human gingival tissue.Virology journal · 2006Article
- Calprotectin expression by gingival epithelial cells.Infection and immunity · 2001Article
- Novel human alpha9 acetylcholine receptor regulating keratinocyte adhesion is targeted by Pemphigus vulgaris autoimmunity.The American journal of pathology · 2000Article
- Reconstituted human oral and esophageal mucosa in culture.In vitro cellular & developmental biology. Animal · 1998Article
- Reconstituted human gingival epithelium: nonsubmerged in vitro model.In vitro cellular & developmental biology. Animal · 1997Article
- Cell, tissue and organ culture as in vitro models to study the biology of squamous cell carcinomas of the head and neck.Cancer metastasis reviews · 1996Review
- Growth regulation of serum-free cultures of epithelial cells from normal human buccal mucosa.In vitro cellular & developmental biology : journal of the Tissue Culture Association · 1991Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Epithelial cells from human fetal and adult gingiva were cultured in keratinocyte growth medium (KGM), a serum-free medium. The expression of keratin proteins in these cells was evaluated using immunohistochemistry and SDS-PAGE-immunoblot analysis and compared with expression in the tissue. Keratins 5, 6, 14, 16, and 19 were identified in cells cultured from both fetal and adult tissues. K19 was localized in basal cells of fetal oral tissue but was not seen in adult gingiva (except for scattered Merkel cells). K1 and K10 were expressed in tissue, but not in cultured cells. The keratin profiles of cultured epithelial cells from several adult donors were similar and were identical in cultures from primary through Passage 5. K13, a differentiation-specific keratin, was expressed in all suprabasal cells of fetal oral epithelium, but shows only spotty expression in adult gingival tissue. K13 was expressed in cultures of fetal cells, but very weakly or not at all in cultures of adult cells. K13 expression was greater in cultures grown with physiologic calcium concentrations (1.2 mM) than in those grown at 0.15 mM or less. Our findings are consistent with basal-like characters of these cells in 0.15 mM calcium growth conditions. Differentiation of fetal oral cells in culture to the suprabasal basal cell stage in 1.2 mM Ca2+ is shown by the expression of K13.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.