ArticleThe EMBO journal1990
Antibody mapping of functional domains in vinculin.
Article in The EMBO journal, 1990. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 63 citations in OpenAlex.
- Talin contains three similar vinculin-binding sites predicted to form an amphipathic helix.The Biochemical journal · 1999Article
- Concerted activity of tyrosine phosphatase SHP-2 and focal adhesion kinase in regulation of cell motility.Molecular and cellular biology · 1999Article
- Disruption of the talin gene compromises focal adhesion assembly in undifferentiated but not differentiated embryonic stem cells.The Journal of cell biology · 1998Article
- Talin and vinculin play distinct roles in filopodial motility in the neuronal growth cone.The Journal of cell biology · 1996Article
- Characterization of an F-actin-binding domain in the cytoskeletal protein vinculin.The Journal of cell biology · 1994Article
- Suppression of vinculin expression by antisense transfection confers changes in cell morphology, motility, and anchorage-dependent growth of 3T3 cells.The Journal of cell biology · 1993Article
- The cytoskeletal protein talin contains at least two distinct vinculin binding domains.The Journal of cell biology · 1993Article
- Expression of chicken vinculin complements the adhesion-defective phenotype of a mutant mouse F9 embryonal carcinoma cell.The Journal of cell biology · 1993Article
- Actin-membrane coupling: a role for talin.Journal of muscle research and cell motility · 1992Article
- The 46/50 kDa phosphoprotein VASP purified from human platelets is a novel protein associated with actin filaments and focal contacts.The EMBO journal · 1992Article
- The uvomorulin-anchorage protein alpha catenin is a vinculin homologue.Proceedings of the National Academy of Sciences of the United States of America · 1991Article
- Intercellular spread of Shigella flexneri through a monolayer mediated by membranous protrusions and associated with reorganization of the cytoskeletal protein vinculin.Infection and immunity · 1991Article
- Antibody mapping of tissue factor implicates two different exon-encoded regions in function.The Biochemical journal · 1991Article
- Actin binding proteins--lipid interactions.Journal of muscle research and cell motility · 1991Review
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We have analyzed the functional domain structure of vinculin, a protein involved in linking microfilaments to the cytoplasmic face of cell membranes in animal cells. For this purpose, we used several monoclonal antibodies raised against chicken gizzard vinculin whose epitopes could be assigned to discrete regions in the vinculin sequence by immunoblotting of proteolytic fragments combined with N-terminal amino acid sequencing. Two of these antibodies induced the disruption of stress fibers and changed the number of morphology of focal contacts after microinjection in chicken embryo fibroblasts. Based on the location of its epitope in comparison with vinculin domains previously identified by other groups, we propose that one of these antibodies (15B7) interferes with the binding of vinculin to talin, the most peripheral of the microfilament proteins. The second antibody (14C10) binds within a region comprising three internal repeats and might therefore distort the inner architecture of vinculin. A third antibody (As3) inhibited the binding of F-actin to vinculin in an in vitro assay but had no effect on the microfilament system in cells. These data emphasize the role of vinculin as a key protein in microfilament-membrane linkage and support previous work on a direct interaction between vinculin and actin.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.