ArticlePlant molecular biology2006
A Gateway-based platform for multigene plant transformation.
Article in Plant molecular biology, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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Who cites it
33 citing papers in PubMed.
- Bxb1 and ParA recombinase-based gene assembly for multiple genes expression in plant cells.Plant physiology · 2026Article
- Pyramiding stacking of multigenes (PSM): a simple, flexible and efficient multigene stacking system based on Gibson assembly and gateway cloning.Frontiers in bioengineering and biotechnology · 2023Article
- Plant Metabolic Engineering by Multigene Stacking: Synthesis of Diverse Mogrosides.International journal of molecular sciences · 2022Article
- Split-Cre mediated deletion of DNA no longer needed after site-specific integration in rice.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2022Article
- Heterologous Biosynthesis of Health-Promoting Baicalein inMolecules (Basel, Switzerland) · 2022Article
- An Efficient Modular Gateway Recombinase-Based Gene Stacking System for Generating Multi-Trait Transgenic Plants.Plants (Basel, Switzerland) · 2022Article
- Site-Specific Sequence Exchange Between Homologous and Non-homologous Chromosomes.Frontiers in plant science · 2022Article
- Developing specific leaf promoters tools for genetic use in transgenic plants towards food security.Saudi journal of biological sciences · 2021Article
- Plant Synthetic Metabolic Engineering for Enhancing Crop Nutritional Quality.Plant communications · 2020Review
- Concurrent Overexpression ofFrontiers in plant science · 2018Article
- Plant X-tender: An extension of the AssemblX system for the assembly and expression of multigene constructs in plants.PloS one · 2018Article
- Co-expression of P173S Mutant RiceFrontiers in plant science · 2018Article
- Plant artificial chromosome technology and its potential application in genetic engineering.Plant biotechnology journal · 2016Review
- Functional conservation and divergence of Miscanthus lutarioriparius GT43 gene family in xylan biosynthesis.BMC plant biology · 2016Article
- A simple and reliable multi-gene transformation method for switchgrass.Plant cell reports · 2014Article
- Creation and validation of a widely applicable multiple gene transfer vector system for stable transformation in plant.Plant molecular biology · 2013Article
- GoldenBraid 2.0: a comprehensive DNA assembly framework for plant synthetic biology.Plant physiology · 2013Article
- Delivery of multiple transgenes to plant cells by an improved version of MultiRound Gateway technology.Transgenic research · 2013Article
- A modified MultiSite gateway cloning strategy for consolidation of genes in plants.Molecular biotechnology · 2013Article
- Expression pattern of the alpha-kafirin promoter coupled with a signal peptide from Sorghum bicolor L. Moench.Journal of biomedicine & biotechnology · 2012Article
Corrections and comments
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The post-genomic era offers unrivalled opportunities for genetic manipulation of polygenic traits, multiple traits, and multiple gene products. However, remaining technical hurdles make the manipulation of multiple genes in plants difficult. Here we describe a Gateway-based vector system to enable multiple transgenes to be directly linked or fused. The vector system consists of a destination vector and two special attL-flanked entry vectors each containing an attR cassette incompatible with the attL. By multiple rounds of LR recombination reactions, which we call MultiRound Gateway, multiple transgenes can be delivered sequentially and indefinitely into the Gateway-compatible destination vector through alternate use of the two special entry vectors. In our proof-of-principle experiments we have used this vector system to construct a plant transformation vector containing seven functional DNA fragments, including a screening marker gene, two reporter genes and four matrix attachment region sequences. This system provides a platform for fully realizing the potential of plant genetic manipulation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.