ArticleJournal of virology2006
High-throughput, library-based selection of a murine leukemia virus variant to infect nondividing cells.
Article in Journal of virology, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
11 citing papers in PubMed, 22 citations in OpenAlex.
- Repression of the Chromatin-Tethering Domain of Murine Leukemia Virus p12.Journal of virology · 2016Article
- Systemic attenuation of the TGF-β pathway by a single drug simultaneously rejuvenates hippocampal neurogenesis and myogenesis in the same old mammal.Oncotarget · 2015Article
- Age-Associated Increase in BMP Signaling Inhibits Hippocampal Neurogenesis.Stem cells (Dayton, Ohio) · 2015Article
- Viral DNA tethering domains complement replication-defective mutations in the p12 protein of MuLV Gag.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- The Gag cleavage product, p12, is a functional constituent of the murine leukemia virus pre-integration complex.PLoS pathogens · 2010Article
- Specific insertions of zinc finger domains into Gag-Pol yield engineered retroviral vectors with selective integration properties.Proceedings of the National Academy of Sciences of the United States of America · 2010Article
- Relative roles of TGF-beta1 and Wnt in the systemic regulation and aging of satellite cell responses.Aging cell · 2009Article
- DNA shuffling of adeno-associated virus yields functionally diverse viral progeny.Molecular therapy : the journal of the American Society of Gene Therapy · 2008Article
- Designer gene delivery vectors: molecular engineering and evolution of adeno-associated viral vectors for enhanced gene transfer.Pharmaceutical research · 2008Review
- Molecular engineering of viral gene delivery vehicles.Annual review of biomedical engineering · 2008Review
- HIV infection of non-dividing cells: a divisive problem.Retrovirology · 2006Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Gammaretroviruses, such as murine leukemia virus (MLV), are functionally distinguished from lentiviruses, such as human immunodeficiency virus, by their inability to infect nondividing cells. Attempts to engineer this property into MLV have been hindered by an incomplete understanding of early events in the viral life cycle. We utilized a transposon-based method to generate saturated peptide insertion libraries of MLV gag-pol variants with nuclear localization signals randomly incorporated throughout these overlapping genes. High-throughput selection of the libraries via iterative retroviral infection of nondividing cells led to the identification of a novel variant that successfully transduced growth-arrested cells. Vector packaging by cotransfection of the gag-pol.NLS variant with wild-type gag-pol produced high-titer virions capable of infecting neurons in vitro and in vivo. The capacity of mutant virions to transduce nondividing cells could help to elucidate incompletely understood mechanisms of the viral life cycle and greatly broaden the gene therapy applications of retroviral vectors. Furthermore, the ability to engineer key intracellular viral infection steps has potential implications for the understanding, design, and control of other post-entry events. Finally, this method of library generation and selection for a desired phenotype directly in a mammalian system can be readily expanded to address other challenges in protein engineering.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.