ArticleGlycobiology2006
Characterization of a carbohydrate epitope defined by the monoclonal antibody H185: sialic acid O-acetylation on epithelial cell-surface mucins.
Article in Glycobiology, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
28 citing papers in PubMed, 46 citations in OpenAlex.
- Profiling O-Acetylation in Sialoglycans Using MALDI-TOF and LC-MS/MS with Methylamidation and Permethylation Derivatization.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Emerging mechanisms in dry eye disease: apoptosis-associated tear MicroRNAs and toxicant-related alterations of the glycocalyx and lipid rafts.Frontiers in ophthalmology · 2026Article
- Ocular surface glycocalyx in health and disease.Frontiers in cell and developmental biology · 2025Review
- Regional Conjunctival Differences in Glycocalyx Mucin Expression in Dry Eye and Normal Subjects.Investigative ophthalmology & visual science · 2024Article
- Quantitative Standards of 4-O-Acetyl- and 9-O-Acetyl-N-Acetylneuraminic Acid for the Analysis of Plasma and Serum.Chembiochem : a European journal of chemical biology · 2022Article
- Human ocular mucins: The endowed guardians of sight.Advanced drug delivery reviews · 2022Review
- Membrane-associated mucins of the human ocular surface in health and disease.The ocular surface · 2021Review
- The ocular surface, coronaviruses and COVID-19.Clinical & experimental optometry · 2020Review
- Disrupted Glycocalyx as a Source of Ocular Surface Biomarkers.Eye & contact lens · 2020Review
- Glycosylation pathways at the ocular surface.Biochemical Society transactions · 2018Review
- TFOS DEWS II Tear Film Report.The ocular surface · 2017Review
- Eukaryotic protein glycosylation: a primer for histochemists and cell biologists.Histochemistry and cell biology · 2017Review
- Human conjunctival goblet cells express the membrane associated mucin MUC16: Localization to mucin granules.Experimental eye research · 2016Article
- Functions and Biosynthesis of O-Acetylated Sialic Acids.Topics in current chemistry · 2015Review
- Comparison of the transmembrane mucins MUC1 and MUC16 in epithelial barrier function.PloS one · 2014Article
- The use of sialidase therapy for respiratory viral infections.Antiviral research · 2013Review
- Glycobiology of the ocular surface: mucins and lectins.Japanese journal of ophthalmology · 2013Review
- Assessing mucin expression and function in human ocular surface epithelia in vivo and in vitro.Methods in molecular biology (Clifton, N.J.) · 2012Article
- Comparison of mucin levels at the ocular surface of postmenopausal women with and without a history of dry eye.Cornea · 2011Article
- Structure and biological roles of mucin-type O-glycans at the ocular surface.The ocular surface · 2010Review
Corrections and comments
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Authors and funding
2 authors at 2 institutions in 1 country.
Funding
Abstract
Sialic acids comprise a large family of derivatives of neuraminic acid containing methyl, acetyl, sulfate, and phosphate among other groups, which confer specific physicochemical properties (e.g., hydrophobicity and resistance to hydrolases) to the molecules carrying them. Several years ago, a monoclonal antibody, designated H185, was developed, which binds to cell membranes of human corneal, conjunctival, laryngeal, and vaginal epithelia and whose distribution is altered on the ocular surface of patients with keratinizing disease. Recent findings using immunoprecipitation and immunodepletion techniques have demonstrated that, in human corneal epithelial cells, the H185 antigen is carried by the membrane-associated mucin MUC16. In this study, we show that the H185 epitope on human corneal cells and in tear fluid is an O-acetylated sialic acid epitope that can be selectively hydrolyzed in an enzyme-concentration-dependent manner by sialidase from Arthrobacter ureafaciens and to a lesser extent by sialidases from Newcastle disease virus, Clostridium perfringens, and Streptococcus pneumoniae. Binding of the H185 antibody was impaired by treatment of tear fluid with a recombinant 9-O-acetylesterase from influenza C virus. Two O-acetyl derivatives, Neu5,7Ac(2) and Neu5,9Ac(2), were identified in human tear fluid by fluorometric high-performance liquid chromatography (HPLC) and electrospray mass spectrometry (MS). Immunoprecipitation of the H185 epitope from human corneal epithelial cells revealed that Neu5,9Ac(2) was the major derivative on the mucin isolate. These results indicate that exposed wet-surfaced epithelia are decorated with O-acetyl sialic acid derivatives on membrane-associated mucins and suggest that O-acetylation on cell surfaces may protect against pathogen infection by preventing degradation of membrane-associated mucins.
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Registered trials
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