ArticleAntimicrobial agents and chemotherapy2006
Mobilizable IncQ-related plasmid carrying a new quinolone resistance gene, qnrS2, isolated from the bacterial community of a wastewater treatment plant.
Article in Antimicrobial agents and chemotherapy, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.
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Who cites it
38 citing papers in PubMed.
- Article
- Bacterial Communities and Resistance and Virulence Genes in Hospital and Community Wastewater: Metagenomic Analysis.International journal of molecular sciences · 2025Article
- Occurrence of antibiotics and bacterial resistance genes in wastewater: resistance mechanisms and antimicrobial resistance control approaches.World journal of microbiology & biotechnology · 2022Review
- Beyond the ABCs-Discovery of Three New Plasmid Types inMicroorganisms · 2022Article
- Public health implications of plasmid-mediated quinolone and aminoglycoside resistance genes inEpidemiology and infection · 2022Article
- High Prevalence of Drug Resistance and Class 1 Integrons inFrontiers in microbiology · 2021Article
- Molecular Characterization of Class 1, 2 and 3 Integrons inInfection and drug resistance · 2021Article
- Molecular Characterization and Comparative Genomics of IncQ-3 Plasmids Conferring Resistance to Various Antibiotics Isolated from a Wastewater Treatment Plant in Warsaw (Poland).Antibiotics (Basel, Switzerland) · 2020Article
- Emerging coexistence of three PMQR genes on a multiple resistance plasmid with a new surrounding genetic structure of qnrS2 in E. coli in China.Antimicrobial resistance and infection control · 2020Article
- Emergence and Characterization of a Novel IncP-6 Plasmid HarboringFrontiers in microbiology · 2019Article
- First Report of an OXA-48- and CTX-M-213-Producing Kluyvera Species Clone Recovered from Patients Admitted in a University Hospital in Madrid, Spain.Antimicrobial agents and chemotherapy · 2018Article
- Comparative Genomic Studies ofFrontiers in microbiology · 2018Article
- Enhanced Bacterial Fitness Under Residual Fluoroquinolone Concentrations Is Associated With Increased Gene Expression in Wastewater-DerivedFrontiers in microbiology · 2018Article
- Characterization of Four Multidrug Resistance Plasmids Captured from the Sediments of an Urban Coastal Wetland.Frontiers in microbiology · 2017Article
- Characterization of Metagenomes in Urban Aquatic Compartments Reveals High Prevalence of Clinically Relevant Antibiotic Resistance Genes in Wastewaters.Frontiers in microbiology · 2017Article
- Article
- Environmental dissemination of antibiotic resistance genes and correlation to anthropogenic contamination with antibiotics.Infection ecology & epidemiology · 2015Article
- Insight into the mobilome of Aeromonas strains.Frontiers in microbiology · 2015Review
- Genomic and Functional Characterization of qnr-Encoding Plasmids from Municipal Wastewater Biosolid Klebsiella pneumoniae Isolates.Frontiers in microbiology · 2015Article
- Plasmid-mediated quinolone resistance.Microbiology spectrum · 2014Review
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4 authors.
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Abstract
Plasmid-encoded quinolone resistance was previously reported for different bacteria isolated from patients not only in the United States and Asia but also in Europe. Here we describe the isolation, by applying a new selection strategy, of the quinolone resistance plasmid pGNB2 from an activated sludge bacterial community of a wastewater treatment plant in Germany. The hypersensitive Escherichia coli strain KAM3 carrying a mutation in the multidrug efflux system genes acrAB was transformed with total plasmid DNA preparations isolated from activated sludge bacteria and subsequently selected on medium containing the fluoroquinolone norfloxacin. This approach resulted in the isolation of plasmid pGNB2 conferring decreased susceptibility to nalidixic acid and to different fluoroquinolones. Analysis of the pGNB2 nucleotide sequence revealed that it is 8,469 bp in size and has a G+C content of 58.2%. The plasmid backbone is composed of a replication initiation module (repA-repC) belonging to the IncQ-family and a two-component mobilization module that confers horizontal mobility to the plasmid. In addition, plasmid pGNB2 carries an accessory module consisting of a transposon Tn1721 remnant and the quinolone resistance gene, qnrS2, that is 92% identical to the qnrS gene located on plasmid pAH0376 from Shigella flexneri 2b. QnrS2 belongs to the pentapeptide repeat protein family and is predicted to protect DNA-gyrase activity against quinolones. This is not only the first report on a completely sequenced plasmid mediating quinolone resistance isolated from an environmental sample but also on the first qnrS-like gene detected in Europe.
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