ArticleProceedings of the National Academy of Sciences of the United States of America2006
Characterization of capsaicin synthase and identification of its gene (csy1) for pungency factor capsaicin in pepper (Capsicum sp.).
Article in Proceedings of the National Academy of Sciences of the United States of America, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It has been retracted, and should not be counted. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed, 60 citations in OpenAlex.
- Total Syntheses of Deuterated Drugs: A Comprehensive Review.Topics in current chemistry (Cham) · 2025Review
- A comprehensive review of capsaicin: Biosynthesis, industrial productions, processing to applications, and clinical uses.Heliyon · 2024Review
- Triple layer hermetic bags for safe storage of dry Chilli (Journal of food science and technology · 2023Article
- MYB24 Negatively Regulates the Biosynthesis of Lignin and Capsaicin by Affecting the Expression of Key Genes in the Phenylpropanoid Metabolism Pathway inMolecules (Basel, Switzerland) · 2023Article
- Discovery of putative capsaicin biosynthetic genes by RNA-Seq and digital gene expression analysis of pepper.Scientific reports · 2016Article
- Chemical and pharmacological aspects of capsaicin.Molecules (Basel, Switzerland) · 2011Review
- A dynamic interface for capsaicinoid systems biology.Plant physiology · 2009Article
- Article
- Domestication of plants in the Americas: insights from Mendelian and molecular genetics.Annals of botany · 2007Review
- Identification, validation and survey of a single nucleotide polymorphism (SNP) associated with pungency in Capsicum spp.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2007Article
Corrections and comments
- Retraction · 2008-12-23Error in Data · Error in Results and/or Conclusions · · BA 5/30/17
- Erratum issued
- Retracted
Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Capsaicin is a unique alkaloid of the plant kingdom restricted to the genus Capsicum. Capsaicin is the pungency factor, a bioactive molecule of food and of medicinal importance. Capsaicin is useful as a counterirritant, antiarthritic, analgesic, antioxidant, and anticancer agent. Capsaicin biosynthesis involves condensation of vanillylamine and 8-methyl nonenoic acid, brought about by capsaicin synthase (CS). We found that CS activity correlated with genotype-specific capsaicin levels. We purified and characterized CS ( approximately 35 kDa). Immunolocalization studies confirmed that CS is specifically localized to the placental tissues of Capsicum fruits. Western blot analysis revealed concomitant enhancement of CS levels and capsaicin accumulation during fruit development. We determined the N-terminal amino acid sequence of purified CS, cloned the CS gene (csy1) and sequenced full-length cDNA (981 bp). The deduced amino acid sequence of CS from full-length cDNA was 38 kDa. Functionality of csy1 through heterologous expression in recombinant Escherichia coli was also demonstrated. Here we report the gene responsible for capsaicin biosynthesis, which is unique to Capsicum spp. With this information on the CS gene, speculation on the gene for pungency is unequivocally resolved. Our findings have implications in the regulation of capsaicin levels in Capsicum genotypes.
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