ArticleWorld journal of gastroenterology2006
HepG2 cells support viral replication and gene expression of hepatitis C virus genotype 4 in vitro.
Article in World journal of gastroenterology, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed, 52 citations in OpenAlex.
- Inhibition of hepatitis C virus replication in HepG2 cells via modulation of the Raf-1 and interferon-alpha signaling pathways by thymoquinone.Archives of virology · 2025Article
- Downregulation of SOCS1 increases interferon-induced ISGylation during differentiation of induced-pluripotent stem cells to hepatocytes.JHEP reports : innovation in hepatology · 2022Article
- Development of latent Interferon alpha 2b as a safe therapeutic for treatment of Hepatitis C virus infection.Scientific reports · 2019Article
- Advances in the role of HCV nonstructural protein 5a (NS5A) of 3a genotype in inducing insulin resistance by possible phosphorylation of AKT/PKB.Scientific reports · 2019Article
- Screening the anti infectivity potentials of native N- and C-lobes derived from the camel lactoferrin against hepatitis C virus.BMC complementary and alternative medicine · 2014Article
- Inhibitory effect of kaolin minerals compound against hepatitis C virus in Huh-7 cell lines.BMC research notes · 2014Article
- Effectiveness of human, camel, bovine and sheep lactoferrin on the hepatitis C virus cellular infectivity: comparison study.Virology journal · 2013Article
- Very-low-density lipoprotein (VLDL)-producing and hepatitis C virus-replicating HepG2 cells secrete no more lipoviroparticles than VLDL-deficient Huh7.5 cells.Journal of virology · 2013Article
- Anti-infectivity of camel polyclonal antibodies against hepatitis C virus in Huh7.5 hepatoma.Virology journal · 2012Article
- Development of persistent HCV genotype 3a infection cell culture model in huh-7 cell.Virology journal · 2012Article
- Inhibition of HCV 3a genotype entry through host CD81 and HCV E2 antibodies.Journal of translational medicine · 2011Article
- HCV entry receptors as potential targets for siRNA-based inhibition of HCV.Genetic vaccines and therapy · 2011Article
- Examination of the activity of camel milk casein against hepatitis C virus (genotype-4a) and its apoptotic potential in hepatoma and hela cell lines.Hepatitis monthly · 2011Article
- Neutralizing activities of caprine antibodies towards conserved regions of the HCV envelope glycoprotein E2.Virology journal · 2011Article
- Characterization of chronic HCV infection-induced apoptosis.Comparative hepatology · 2011Article
- Glycyrrhizin as antiviral agent against Hepatitis C Virus.Journal of translational medicine · 2011Article
- Effect of combined siRNA of HCV E2 gene and HCV receptors against HCV.Virology journal · 2011Article
- Down-regulation of IRES containing 5'UTR of HCV genotype 3a using siRNAs.Virology journal · 2011Article
- Antiviral activity of Acacia nilotica against Hepatitis C Virus in liver infected cells.Virology journal · 2011Article
- Inhibition of full length hepatitis C virus particles of 1a genotype through small interference RNA.Virology journal · 2011Article
Corrections and comments
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Authors and funding
12 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
aimTo establish a cell culture system with long-term replication of hepatitis C virus (HCV) genome and expression of viral antigens in vitro.
methodsHepG2 cell line was tested for its susceptibility to HCV by incubation with a serum from a patient with chronic hepatitis C. Cells and supernatant were harvested at various time points during the culture. Culture supernatant was tested for its ability to infect naive cells. The presence of minus (antisense) RNA strand, and the detection of core and E1 antigens in cells were examined by RT-PCR and immunological techniques (flow cytometry and Western blot) respectively.
resultsThe intracellular HCV RNA was first detected on d 3 after infection and then could be consistently detected in both cells and supernatant over a period of at least three months. The fresh cells could be infected with supernatant from cultured infected cells. Flow cytometric analysis showed surface and intracellular HCV antigen expression using in house made polyclonal antibodies (anti-core, and anti-E1). Western blot analysis showed the expression of a cluster of immunogenic peptides at molecular weights extended between 31 and 45 kDa in an one month old culture of infected cells whereas this cluster was undetectable in uninfected HepG2 cells.
conclusionHepG2 cell line is not only susceptible to HCV infection but also supports its replication in vitro. Expression of HCV structural proteins can be detected in infected HepG2 cells. These cells are also capable of shedding viral particles into culture media which in turn become infectious to uninfected cells.
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