ArticleMedical & biological engineering & computing2006
Inhibition of cardiomyocyte automaticity by electrotonic application of inward rectifier current from Kir2.1 expressing cells.
Article in Medical & biological engineering & computing, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 34 citations in OpenAlex.
- The network of cardiac KNaunyn-Schmiedeberg's archives of pharmacology · 2024Review
- Chronic Propafenone Application Increases Functional KPharmaceuticals (Basel, Switzerland) · 2023Article
- Electrophysiology of hiPSC-Cardiomyocytes Co-Cultured with HEK Cells Expressing the Inward Rectifier Channel.International journal of molecular sciences · 2021Article
- Quantitative Analysis of the Cytoskeleton's Role in Inward Rectifier KFrontiers in physiology · 2021Article
- LUF7244, an allosteric modulator/activator of KBritish journal of pharmacology · 2019Article
- Generation and customization of biosynthetic excitable tissues for electrophysiological studies and cell-based therapies.Nature protocols · 2018Article
- Class III antiarrhythmic drugs amiodarone and dronedarone impair KJournal of cellular and molecular medicine · 2017Article
- Phase transitions in the multi-cellular regulatory behavior of pancreatic islet excitability.PLoS computational biology · 2014Article
- Structure-activity relationships of pentamidine-affected ion channel trafficking and dofetilide mediated rescue.British journal of pharmacology · 2013Article
- Inhibiting the clathrin-mediated endocytosis pathway rescues K(IR)2.1 downregulation by pentamidine.Pflugers Archiv : European journal of physiology · 2013Article
- Genetic engineering of somatic cells to study and improve cardiac function.Europace : European pacing, arrhythmias, and cardiac electrophysiology : journal of the working groups on cardiac pacing, arrhythmias, and cardiac cellular electrophysiology of the European Society of Cardiology · 2012Article
- Human cardiomyocyte progenitor cells: a short history of nearly everything.Journal of cellular and molecular medicine · 2012Review
- Size and ionic currents of unexcitable cells coupled to cardiomyocytes distinctly modulate cardiac action potential shape and pacemaking activity in micropatterned cell pairs.Circulation. Arrhythmia and electrophysiology · 2012Article
- Cell and gene therapy for arrhythmias: Repair of cardiac conduction damage.Journal of geriatric cardiology : JGC · 2011Article
- Regenerative therapies in electrophysiology and pacing: introducing the next steps.Journal of interventional cardiac electrophysiology : an international journal of arrhythmias and pacing · 2011Review
- Engineering biosynthetic excitable tissues from unexcitable cells for electrophysiological and cell therapy studies.Nature communications · 2011Article
- Hyperpolarization induces differentiation in human cardiomyocyte progenitor cells.Stem cell reviews and reports · 2010Article
- Foetal and adult cardiomyocyte progenitor cells have different developmental potential.Journal of cellular and molecular medicine · 2010Article
- The anti-protozoal drug pentamidine blocks KIR2.x-mediated inward rectifier current by entering the cytoplasmic pore region of the channel.British journal of pharmacology · 2010Article
- Computer modelling of the sinoatrial node.Medical & biological engineering & computing · 2007Review
Corrections and comments
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Authors and funding
8 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A biological pacemaker might be created by generation of a cellular construct consisting of cardiac cells that display spontaneous membrane depolarization, and that are electrotonically coupled to surrounding myocardial cells by means of gap junctions. Depending on the frequency of the spontaneously beating cells, frequency regulation might be required. We hypothesized that application of Kir2.1 expressing non-cardiac cells, which provide I (K1) to spontaneously active neonatal cardiomyocytes (NCMs) by electrotonic coupling in such a cellular construct, would generate an opportunity for pacemaker frequency control. Non-cardiac Kir2.1 expressing cells were co-cultured with spontaneously active rat NCMs. Electrotonic coupling between the two cell types resulted in hyperpolarization of the cardiomyocyte membrane potential and silencing of spontaneous activity. Either blocking of gap-junctional communication by halothane or inhibition of I (K1) by BaCl(2) restored the original membrane potential and spontaneous activity of the NCMs. Our results demonstrate the power of electrotonic coupling for the application of specific ion currents into an engineered cellular construct such as a biological pacemaker.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.