ArticleHistochemistry1990
New sensitive light microscopical detection of colloidal gold on ultrathin sections by reflection contrast microscopy. Combination of reflection contrast and electron microscopy in post-embedding immunogold histochemistry.
Article in Histochemistry, 1990. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 35 citations in OpenAlex.
- A Confocal Reflection Super-Resolution Technique to Image Golgi-Cox Stained Neurons.Journal of microscopy · 2019Article
- Immunogold labelling of neuroendocrine peptides with special reference to antibody specificity and multiple staining techniques.Histochemistry and cell biology · 1996Review
- Cytochemical detection systems for in situ hybridization, and the combination with immunocytochemistry, 'who is still afraid of red, green and blue?'.The Histochemical journal · 1995Review
- Autoantibodies to the laminin P1 fragment in HgCl2-induced membranous glomerulopathy.The American journal of pathology · 1995Article
- Combined analysis of in situ hybridization, cell cycle and structural markers using reflectance and immunofluorescence confocal microscopy.The Histochemical journal · 1995Article
- Reflection contrast microscopy within chrome-alum haematoxylin stained thick tissue-sections.Histochemistry · 1994Article
- Multicolour preparations for in situ hybridization using precipitating enzyme cytochemistry in combination with reflection contrast microscopy.Histochemistry · 1993Article
- Ferritin accumulation and uroporphyrin crystal formation in hepatocytes of C57BL/10 mice: a time-course study.Cell and tissue research · 1993Article
- Reflection contrast microscopy of ultrathin sections in immunocytochemical localization studies: a versatile technique bridging electron microscopy with light microscopy.Histochemistry · 1993Article
- Epitope specificity of anti-gp330 autoantibodies determines the development of proteinuria in active Heymann nephritis.The American journal of pathology · 1993Article
- A histologic study of the extracellular matrix during the development of glomerulosclerosis in murine chronic graft-versus-host disease.The American journal of pathology · 1992Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
One simple post-embedding method for combined light- and electron microscopy is presented. Different types of antigens in normal rat and mouse kidneys as well as in tissues from cases of experimental induced nephritis were stained after Lowicryl K4M embedding by an immunogold (silver) method. The (silver-enhanced) gold particles were visualized by light microscopy, e.g. bright-field (BFM)- and reflection contrast (RCM) microscopy, as well as by electron microscopy. The potentials of RCM visualization in this field were investigated, resulting in the successful detection of colloidal gold (15 nm) particles, or silver enhanced gold particles, on ultrathin sections. Furthermore, an increased detection sensitivity of RCM compared with BFM together with an increase in the sensitivity of the immunostaining by RCM visualization was found. The different ways to use RCM, alone or in combination with bright-field- or phase contrast microscopy for visualization of plastic sections varying in thickness, type of plastic and staining, are discussed.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.