ArticleHistochemistry1990
Lectin--digoxigenin conjugates: a new hapten system for glycoconjugate cytochemistry.
Article in Histochemistry, 1990. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
26 citing papers in PubMed, 71 citations in OpenAlex.
- Clinical Proteomics Profiling for Biomarker Identification Among Patients Suffering With Indian Post Kala Azar Dermal Leishmaniasis.Frontiers in cellular and infection microbiology · 2020Article
- Article
- Lectins for histochemical demonstration of glycans.Histochemistry and cell biology · 2011Review
- Spatiotemporal expression patterns of sialoglycoconjugates during nephron morphogenesis and their regional and cell type-specific distribution in adult rat kidney.Histochemistry and cell biology · 2003Article
- Glycan composition of follicle (Sertoli) cells of the amphibian Pleurodeles waltl. A lectin histochemical study.Journal of anatomy · 2001Article
- Carbohydrate moieties of the interstitial and glandular tissues of the amphibian Pleurodeles waltl testis shown by lectin histochemistry.Journal of anatomy · 2001Article
- Lectin histochemical identification of the carbohydrate moieties on N- and O-linked oligosaccharides in the duct cells of the testis of an amphibian urodele, the spanish newt (Pleurodeles waltl).The Histochemical journal · 2000Article
- Lectin cytochemical characterization of the N- and O-linked oligosaccharides in the human rectum.The Histochemical journal · 2000Article
- Lectin histochemical localization of N- and O-linked oligosaccharides during the spermiogenesis of the urodele amphibian Pleurodeles waltl.Glycoconjugate journal · 1999Article
- Can malignancy in insulinoma be predicted by the expression patterns of beta 1,6 branching of asparagine-linked oligosaccharides and polysialic acid of the neural cell adhesion molecule?Virchows Archiv : an international journal of pathology · 1996Article
- Protein glycosylation in the endoplasmic reticulum and the Golgi apparatus and cell type-specificity of cell surface glycoconjugate expression: analysis by the protein A-gold and lectin-gold techniques.Histochemistry and cell biology · 1996Review
- Applications of immunogold and lectin-gold labeling in tumor research and diagnosis.Histochemistry and cell biology · 1996Review
- Digoxigenylated primary antibodies for sensitive dual-peroxidase labelling of neural markers.Histochemistry and cell biology · 1995Article
- The substantia nigra is a major target for neurovirulent influenza A virus.The Journal of experimental medicine · 1995Article
- Differential expression of cell surface sialoglycoconjugates on wild-type and cultured Ehrlich tumor cells as revealed by quantitative lectin-gold ultrastructural cytochemistry.Proceedings of the National Academy of Sciences of the United States of America · 1994Article
- Cytochemical staining for beta 1,6 branching of asparagine-linked oligosaccharides in variants of metastatic human colon carcinoma cells.The American journal of pathology · 1994Article
- Subcellular characterization of glycoproteins in the principal cells of human gallbladder. A lectin cytochemical study.Histochemistry · 1994Article
- Use of Phaseolus vulgaris leukoagglutinating lectin in histochemical and blotting techniques: a comparison of digoxigenin- and biotin-labelled lectins.Histochemistry · 1993Article
- Cellular sialoglycoconjugates: a histochemical perspective.The Histochemical journal · 1993Review
- Staining of pancreatic centroacinar cells, liver bile canaliculi and testicular Leydig cells with a monoclonal antibody against adrenocortical cells.Cell and tissue research · 1993Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We report the use of a novel hapten system for lectin cytochemistry. Various lectins conjugated to the steroid hapten digoxigenin (DIG) and monospecific anti-digoxigenin antibodies were applied for the light and electron microscopic detection of glycoconjugates in tissue sections. Both IgG and Fab' anti-DIG antibodies were complexed to particles of colloidal gold and compared to commercially available alkaline phosphatase and horseradish peroxidase conjugated Fab' as general second step reagents. The three different markers performed equally well on paraffin sections whereas the gold-labeled antibodies were superior reagents for semithin and ultrathin sections of Lowicryl K4M embedded tissues. In conjunction with the latter marker, no pretreatment to abolish endogenous enzyme activity was necessary. At the light microscope level, gold signal amplification by the photochemical silver reaction was required. DIG, in contrast to biotin, does not occur in animal tissues thus eliminating the need for blocking reactions prior to lectin incubation. Compared to affinity techniques using glycoprotein-gold complexes as second step reagent the DIG hapten system required smaller amounts of lectins. The staining patterns were indistinguishable from those obtained in other lectin-gold techniques and the specificity of the labeling could be demonstrated in sugar inhibition tests.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.