ArticleJournal of bacteriology2006
Differential telomere processing by Borrelia telomere resolvases in vitro but not in vivo.
Article in Journal of bacteriology, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 39 citations in OpenAlex.
- Structure analysis of the telomere resolvase from the Lyme disease spirochete Borrelia garinii reveals functional divergence of its C-terminal domain.Nucleic acids research · 2024Article
- Characterization and genomic analysis of the Lyme disease spirochete bacteriophage ϕBB-1.PLoS pathogens · 2024Article
- Characterization and genomic analysis of the Lyme disease spirochete bacteriophage ϕBB-1.bioRxiv : the preprint server for biology · 2024Article
- Choreography of Lyme Disease Spirochete Adhesins To Promote Vascular Escape.Microbiology spectrum · 2023Article
- Construction and characterization of a Borrelia burgdorferi strain with conditional expression of the essential telomere resolvase, ResT.Journal of bacteriology · 2014Article
- Peaceful coexistence amongst Borrelia plasmids: getting by with a little help from their friends?Plasmid · 2013Review
- The nucleotide excision repair system of Borrelia burgdorferi is the sole pathway involved in repair of DNA damage by UV light.Journal of bacteriology · 2013Article
- Vascular binding of a pathogen under shear force through mechanistically distinct sequential interactions with host macromolecules.Molecular microbiology · 2012Article
- Altered murine tissue colonization by Borrelia burgdorferi following targeted deletion of linear plasmid 17-carried genes.Infection and immunity · 2012Article
- Preventing broken Borrelia telomeres: ResT couples dual hairpin telomere formation with product release.The Journal of biological chemistry · 2010Article
- Investigation of the genes involved in antigenic switching at the vlsE locus in Borrelia burgdorferi: an essential role for the RuvAB branch migrase.PLoS pathogens · 2009Article
- GuaA and GuaB are essential for Borrelia burgdorferi survival in the tick-mouse infection cycle.Journal of bacteriology · 2009Article
- High-throughput screening identifies three inhibitor classes of the telomere resolvase from the lyme disease spirochete.Antimicrobial agents and chemotherapy · 2009Article
- Identification of the determinant conferring permissive substrate usage in the telomere resolvase, ResT.The Journal of biological chemistry · 2009Article
- Characterization and in vitro reaction properties of 19 unique hairpin telomeres from the linear plasmids of the lyme disease spirochete.The Journal of biological chemistry · 2009Article
- Molecular mechanisms involved in vascular interactions of the Lyme disease pathogen in a living host.PLoS pathogens · 2008Article
- The genome of Cyanothece 51142, a unicellular diazotrophic cyanobacterium important in the marine nitrogen cycle.Proceedings of the National Academy of Sciences of the United States of America · 2008Article
- Real-time high resolution 3D imaging of the lyme disease spirochete adhering to and escaping from the vasculature of a living host.PLoS pathogens · 2008Article
- Purification and properties of the plasmid maintenance proteins from the Borrelia burgdorferi linear plasmid lp17.Journal of bacteriology · 2008Article
- Spent culture medium from virulent Borrelia burgdorferi increases permeability of individually perfused microvessels of rat mesentery.PloS one · 2008Article
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Authors and funding
8 authors at 5 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Causative agents of Lyme disease and relapsing fever, including Borrelia burgdorferi and Borrelia hermsii, respectively, are unusual among bacteria in that they possess a segmented genome with linear DNA molecules terminated by hairpin ends, known as telomeres. During replication, these telomeres are processed by the essential telomere resolvase, ResT, in a unique biochemical reaction known as telomere resolution. In this study, we report the identification of the B. hermsii resT gene through cross-species hybridization. Sequence comparison of the B. hermsii protein with the B. burgdorferi orthologue revealed 67% identity, including all the regions currently known to be crucial for telomere resolution. In vitro studies, however, indicated that B. hermsii ResT was unable to process a replicated B. burgdorferi type 2 telomere substrate. In contrast, in vivo cross-species complementation in which the native resT gene of B. burgdorferi was replaced with B. hermsii resT had no discernible effect, even though B. burgdorferi strain B31 carries at least two type 2 telomere ends. The B. burgdorferi ResT protein was also able to process two telomere spacing mutants in vivo that were unresolvable in vitro. The unexpected differential telomere processing in vivo versus in vitro by the two telomere resolvases suggests the presence of one or more accessory factors in vivo that are normally involved in the reaction. Our current results are also expected to facilitate further studies into ResT structure and function, including possible interaction with other Borrelia proteins.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.