ArticleJournal of bacteriology2006
Localization of the transglutaminase cross-linking sites in the Bacillus subtilis spore coat protein GerQ.
Article in Journal of bacteriology, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed.
- Recent progress in proteins regulating the germination ofJournal of bacteriology · 2025Review
- Molecular architecture of the assembly of Bacillus spore coat protein GerQ revealed by cryo-EM.Nature communications · 2024Article
- Efficient biosynthesis of transglutaminase inCurrent research in food science · 2024Article
- Identification of Native Cross-Links inJournal of proteome research · 2021Article
- Diversity and evolutionary dynamics of spore-coat proteins in spore-forming species of Bacillales.Microbial genomics · 2020Article
- Temporal and spatial regulation of protein cross-linking by the pre-assembled substrates of a Bacillus subtilis spore coat transglutaminase.PLoS genetics · 2019Article
- IL-1 Fragment Modulates Immune Response Elicited by Recombinant Bacillus subtilis Spores Presenting an Antigen/Adjuvant Chimeric Protein.Molecular biotechnology · 2018Article
- Autoregulation of SafA Assembly through Recruitment of a Protein Cross-Linking Enzyme.Journal of bacteriology · 2018Article
- Stoichiometry, Absolute Abundance, and Localization of Proteins in the Bacillus cereus Spore Coat Insoluble Fraction Determined Using a QconCAT Approach.Journal of proteome research · 2018Article
- Spirochaete flagella hook proteins self-catalyse a lysinoalanine covalent crosslink for motility.Nature microbiology · 2016Article
- Article
- Expression and display of a novel thermostable esterase from Clostridium thermocellum on the surface of Bacillus subtilis using the CotB anchor protein.Journal of industrial microbiology & biotechnology · 2015Article
- A system of vectors for Bacillus subtilis spore surface display.Microbial cell factories · 2014Article
- Mucosal adjuvant activity of IL-2 presenting spores of bacillus subtilis in a murine model of Helicobacter pylori vaccination.PloS one · 2014Article
- Article
- New stable anchor protein and peptide linker suitable for successful spore surface display in B. subtilis.Microbial cell factories · 2013Article
- Article
- Expression and display of UreA of Helicobacter acinonychis on the surface of Bacillus subtilis spores.Microbial cell factories · 2010Article
- Roles of the Bacillus anthracis spore protein ExsK in exosporium maturation and germination.Journal of bacteriology · 2009Article
- Protozoal digestion of coat-defective Bacillus subtilis spores produces "rinds" composed of insoluble coat protein.Applied and environmental microbiology · 2008Article
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Authors and funding
2 authors.
Funding
Abstract
The Bacillus subtilis spore coat protein GerQ is necessary for the proper localization of CwlJ, an enzyme important in the hydrolysis of the peptidoglycan cortex during spore germination. GerQ is cross-linked into high-molecular-mass complexes in the spore coat late in sporulation, and this cross-linking is largely due to a transglutaminase. This enzyme forms an epsilon-(gamma-glutamyl) lysine isopeptide bond between a lysine donor from one protein and a glutamine acceptor from another protein. In the current work, we have identified the residues in GerQ that are essential for transglutaminase-mediated cross-linking. We show that GerQ is a lysine donor and that any one of three lysine residues near the amino terminus of the protein (K2, K4, or K5) is necessary to form cross-links with binding partners in the spore coat. This leads to the conclusion that all Tgl-dependent GerQ cross-linking takes place via these three lysine residues. However, while the presence of any of these three lysine residues is essential for GerQ cross-linking, they are not essential for the function of GerQ in CwlJ localization.
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