ArticleJournal of bacteriology2006
The Escherichia coli K-12 NarL and NarP proteins insulate the nrf promoter from the effects of integration host factor.
Article in Journal of bacteriology, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 31 citations in OpenAlex.
- Tyrosol blocks E. coli anaerobic biofilm formation via YbfA and FNR to increase antibiotic susceptibility.Nature communications · 2024Article
- Synergistic interactions between anammox and dissimilatory nitrate reducing bacteria sustains reactor performance across variable nitrogen loading ratios.Frontiers in microbiology · 2023Article
- The Small DdrR Protein Directly Interacts with the UmuDAb Regulator of the Mutagenic DNA Damage Response in Acinetobacter baumannii.Journal of bacteriology · 2022Article
- Article
- Activation by NarL at the Escherichia coli ogt promoter.The Biochemical journal · 2020Article
- Lytic gene expression in the temperate bacteriophage GIL01 is activated by a phage-encoded LexA homologue.Nucleic acids research · 2018Article
- Regulation of nrf operon expression in pathogenic enteric bacteria: sequence divergence reveals new regulatory complexity.Molecular microbiology · 2017Article
- Mlc is a transcriptional activator with a key role in integrating cyclic AMP receptor protein and integration host factor regulation of leukotoxin RNA synthesis in Aggregatibacter actinomycetemcomitans.Journal of bacteriology · 2013Article
- A proteomic analysis of the regulon of the NarP two-component regulatory system response regulator in the bovine pathogen Mannheimia haemolytica A1.BMC research notes · 2011Article
- Repression of aerobic leukotoxin transcription by integration host factor in Aggregatibacter actinomycetemcomitans.Research in microbiology · 2010Article
- Down-regulation of the Escherichia coli K-12 nrf promoter by binding of the NsrR nitric oxide-sensing transcription repressor to an upstream site.Journal of bacteriology · 2010Article
- Regulation by nucleoid-associated proteins at the Escherichia coli nir operon promoter.Journal of bacteriology · 2008Article
- Activation of yeaR-yoaG operon transcription by the nitrate-responsive regulator NarL is independent of oxygen- responsive regulator Fnr in Escherichia coli K-12.Journal of bacteriology · 2007Article
- Recent advances in the expression, evolution, and dynamics of prokaryotic genomes.Journal of bacteriology · 2007Article
- The NsrR regulon of Escherichia coli K-12 includes genes encoding the hybrid cluster protein and the periplasmic, respiratory nitrite reductase.Journal of bacteriology · 2007Article
- Antirepression as a second mechanism of transcriptional activation by a minor groove binding protein.Molecular microbiology · 2007Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
Abstract
The Escherichia coli K-12 nrf operon promoter can be activated fully by the FNR protein (regulator of fumarate and nitrate reduction) binding to a site centered at position -41.5. FNR-dependent transcription is suppressed by integration host factor (IHF) binding at position -54, and this suppression is counteracted by binding of the NarL or NarP response regulator at position -74.5. The E. coli acs gene is transcribed from a divergent promoter upstream from the nrf operon promoter. Transcription from the major acsP2 promoter is dependent on the cyclic AMP receptor protein and is modulated by IHF and Fis binding at multiple sites. We show that IHF binding to one of these sites, located at position -127 with respect to the nrf promoter, has a positive effect on nrf promoter activity. This activation is dependent on the face of the DNA helix, independent of IHF binding at other locations, and found only when NarL/NarP are not bound at position -74.5. Binding of NarL/NarP appears to insulate the nrf promoter from the effects of IHF. The acs-nrf regulatory region is conserved in other pathogenic E. coli strains and related enteric bacteria but differs in Salmonella enterica serovar Typhimurium.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.