ArticleThe Plant cell2006
Overexpression of the Arabidopsis syntaxin PEP12/SYP21 inhibits transport from the prevacuolar compartment to the lytic vacuole in vivo.
Article in The Plant cell, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.
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Who cites it
39 citing papers in PubMed, 88 citations in OpenAlex.
- The clues offered by SNAREs on the vacuoles of plants and animals.Frontiers in plant science · 2025Review
- Cellular and physiological functions of SGR family in gravitropic response in higher plants.Journal of advanced research · 2025Review
- The K/HDEL receptor does not recycle but instead acts as a Golgi-gatekeeper.Nature communications · 2023Article
- Vacuolar membrane structures and their roles in plant-pathogen interactions.Plant molecular biology · 2019Review
- Unusual Roles of Secretory SNARE SYP132 in Plasma Membrane HPlant physiology · 2019Article
- Glycosylphosphatidylinositol-Anchored Proteins inFrontiers in plant science · 2019Review
- Manipulating Endoplasmic Reticulum-Plasma Membrane Tethering in Plants Through Fluorescent Protein Complementation.Frontiers in plant science · 2019Article
- Microtubules play a role in trafficking prevacuolar compartments to vacuoles in tobacco pollen tubes.Open biology · 2018Article
- Predominant Golgi Residency of the Plant K/HDEL Receptor Is Essential for Its Function in Mediating ER Retention.The Plant cell · 2018Article
- Novel fluorochromes label tonoplast in living plant cells and reveal changes in vacuolar organization after treatment with protein phosphatase inhibitors.Protoplasma · 2018Article
- Nanobody-triggered lockdown of VSRs reveals ligand reloading in the Golgi.Nature communications · 2018Article
- Map-based Cloning and Characterization of the BPH18 Gene from Wild Rice Conferring Resistance to Brown Planthopper (BPH) Insect Pest.Scientific reports · 2016Article
- The Arabidopsis Vacuolar Sorting Receptor1 is required for osmotic stress-induced abscisic acid biosynthesis.Plant physiology · 2015Article
- Golgi-dependent transport of vacuolar sorting receptors is regulated by COPII, AP1, and AP4 protein complexes in tobacco.The Plant cell · 2014Article
- Recovery from heat, salt and osmotic stress in Physcomitrella patens requires a functional small heat shock protein PpHsp16.4.BMC plant biology · 2013Article
- Toxoplasma gondii Syntaxin 6 is required for vesicular transport between endosomal-like compartments and the Golgi complex.Traffic (Copenhagen, Denmark) · 2013Article
- In vivo intracellular pH measurements in tobacco and Arabidopsis reveal an unexpected pH gradient in the endomembrane system.The Plant cell · 2013Article
- Identification of key amino acids for the evolution of promoter target specificity of anthocyanin and proanthocyanidin regulating MYB factors.Plant molecular biology · 2013Article
- Defining new SNARE functions: the i-SNARE.Frontiers in plant science · 2013Article
- Trafficking of vacuolar proteins: the crucial role of Arabidopsis vacuolar protein sorting 29 in recycling vacuolar sorting receptor.The Plant cell · 2012Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Golgi-mediated transport to the lytic vacuole involves passage through the prevacuolar compartment (PVC), but little is known about how vacuolar proteins exit the PVC. We show that this last step is inhibited by overexpression of Arabidopsis thaliana syntaxin PEP12/SYP21, causing an accumulation of soluble and membrane cargo and the plant vacuolar sorting receptor BP80 in the PVC. Anterograde transport proceeds normally from the endoplasmic reticulum to the Golgi and the PVC, although export from the PVC appears to be compromised, affecting both anterograde membrane flow to the vacuole and the recycling route of BP80 to the Golgi. However, Golgi-mediated transport of soluble and membrane cargo toward the plasma membrane is not affected, but a soluble BP80 ligand is partially mis-sorted to the culture medium. We also observe clustering of individual PVC bodies that move together and possibly fuse with each other, forming enlarged compartments. We conclude that PEP12/SYP21 overexpression specifically inhibits export from the PVC without affecting the Golgi complex or compromising the secretory branch of the endomembrane system. The results provide a functional in vivo assay that confirms PEP12/SYP21 involvement in vacuolar sorting and indicates that excess of this syntaxin in the PVC can be detrimental for further transport from this organelle.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.