ArticleThe Plant cell2006
Rapid metabolism of glucose detected with FRET glucose nanosensors in epidermal cells and intact roots of Arabidopsis RNA-silencing mutants.
Article in The Plant cell, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 79 papers.
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79 citing papers in PubMed, 196 citations in OpenAlex.
- A Far-Red FRET biosensor for AMPK enables multiplexed imaging of single-cell bioenergetic homeostasis.bioRxiv : the preprint server for biology · 2026Article
- Resolving subcellular sucrose concentrations in plant tissues.Journal of experimental botany · 2026Review
- Evidence for rapid hydrolysis of shoot-derived sucrose using an ultrasensitive ratiometric matryoshka-type MGlucoMeter sensor.The Plant journal : for cell and molecular biology · 2026Article
- Unravelling molecular dynamics in living cells: Fluorescent protein biosensors for cell biology.Journal of microscopy · 2025Review
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- A Monochromatically Excitable Green-Red Dual-Fluorophore Fusion Incorporating a New Large Stokes Shift Fluorescent Protein.Biochemistry · 2024Article
- Review
- Fluorescent Biosensor Imaging of Nitrate inBio-protocol · 2023Article
- Molecular Level Sucrose Quantification: A Critical Review.Sensors (Basel, Switzerland) · 2022Review
- In vivo visualization of nitrate dynamics using a genetically encoded fluorescent biosensor.Science advances · 2022Article
- Nanosensor Applications in Plant Science.Biosensors · 2022Review
- Concept of Fluorescent Transport Activity Biosensor for the Characterization of the Arabidopsis NPF1.3 Activity of Nitrate.Sensors (Basel, Switzerland) · 2022Article
- Recent Progress in Nanobiosensors for Precise Detection of Blood Glucose Level.Biochemistry research international · 2022Review
- Designs, applications, and limitations of genetically encoded fluorescent sensors to explore plant biology.Plant physiology · 2021Review
- DiurnaliScience · 2021Article
- Sensors for the quantification, localization and analysis of the dynamics of plant hormones.The Plant journal : for cell and molecular biology · 2021Review
- Affinity Purification of GO-Matryoshka Biosensors fromBio-protocol · 2020Article
- Live Imaging of Phosphate Levels in Arabidopsis Root Cells Expressing a FRET-Based Phosphate Sensor.Plants (Basel, Switzerland) · 2020Article
- Multiplexed Optical Sensors in Arrayed Islands of Cells for multimodal recordings of cellular physiology.Nature communications · 2020Article
- Fluorescent Biosensors for Neuronal Metabolism and the Challenges of Quantitation.Current opinion in neurobiology · 2020Review
19 more citing papers are in PubMed but not listed here.
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Genetically encoded glucose nanosensors have been used to measure steady state glucose levels in mammalian cytosol, nuclei, and endoplasmic reticulum. Unfortunately, the same nanosensors in Arabidopsis thaliana transformants manifested transgene silencing and undetectable fluorescence resonance energy transfer changes. Expressing nanosensors in sgs3 and rdr6 transgene silencing mutants eliminated silencing and resulted in high fluorescence levels. To measure glucose changes over a wide range (nanomolar to millimolar), nanosensors with higher signal-to-noise ratios were expressed in these mutants. Perfusion of leaf epidermis with glucose led to concentration-dependent ratio changes for nanosensors with in vitro K(d) values of 600 microM (FLIPglu-600 microDelta13) and 3.2 mM (FLIPglu-3.2 mDelta13), but one with 170 nM K(d) (FLIPglu-170 nDelta13) showed no response. In intact roots, FLIPglu-3.2 mDelta13 gave no response, whereas FLIPglu-600 microDelta13, FLIPglu-2 microDelta13, and FLIPglu-170 nDelta13 all responded to glucose. These results demonstrate that cytosolic steady state glucose levels depend on external supply in both leaves and roots, but under the conditions tested they are lower in root versus epidermal and guard cells. Without photosynthesis and external supply, cytosolic glucose can decrease to <90 nM in root cells. Thus, observed gradients are steeper than expected, and steady state levels do not appear subject to tight homeostatic control. Nanosensor-expressing plants can be used to assess glucose flux differences between cells, invertase-mediated sucrose hydrolysis in vivo, delivery of assimilates to roots, and glucose flux in mutants affected in sugar transport, metabolism, and signaling.
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