ArticleBiophysical journal2006
Measurement of monomer-oligomer distributions via fluorescence moment image analysis.
Article in Biophysical journal, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 37 citations in OpenAlex.
- Measuring G protein activation by spectrally resolved imaging fluorescence fluctuation spectroscopy.Biophysical journal · 2025Article
- Multiple allostery in the regulation of PDGFR beta kinase activities.Acta biochimica et biophysica Sinica · 2024Article
- Uptake quantification of gold nanoparticles inside of cancer cells using high order image correlation spectroscopy.Biomedical optics express · 2021Article
- Two-Color Spatial Cumulant Analysis Detects Heteromeric Interactions between Membrane Proteins.Biophysical journal · 2019Article
- Genetically encoded tags for real time dissection of protein assembly in living cells.Chemical science · 2018Article
- An intermolecular FRET sensor detects the dynamics of T cell receptor clustering.Nature communications · 2017Article
- Confocal Spectroscopy to Study Dimerization, Oligomerization and Aggregation of Proteins: A Practical Guide.International journal of molecular sciences · 2016Review
- Quantifying the dynamics of the oligomeric transcription factor STAT3 by pair correlation of molecular brightness.Nature communications · 2016Article
- Aggregation distributions on cells determined by photobleaching image correlation spectroscopy.Biophysical journal · 2013Article
- An elegant way to quantitatively analyze oligomer formation in solution.Biophysical journal · 2012Article
- Review
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- Live-cell visualization of dynamics of HIV budding site interactions with an ESCRT component.Nature cell biology · 2011Article
- Heterospecies partition analysis reveals binding curve and stoichiometry of protein interactions in living cells.Proceedings of the National Academy of Sciences of the United States of America · 2010Article
- Total internal reflection with fluorescence correlation spectroscopy: Applications to substrate-supported planar membranes.Journal of structural biology · 2009Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We present higher-order moment analysis of fluorescence intensity fluctuations from individual laser scanning microscopy images applied to study monomer-oligomer distributions. We demonstrate that the number densities and brightness ratios of a mixed population of monomers and oligomers can be determined by analyzing higher-order moments of the fluorescence intensity fluctuations from individual images for specific ranges of densities and particle brightness ratios. Computer simulations and experiments with fluorescent microspheres and cells were performed to illustrate the detection limits and accuracy of this statistical approach. The simulation results show that the concentration of the dimer or oligomer population should be less than or equal to the monomeric concentration for the method to provide accurate results, and that the upper density detection limit of the population of monomers is one order-of-magnitude higher than the concentration of the oligomers. We implemented this technique to resolve two populations of fluorescent microspheres with different brightness ratios and we also applied the moment-analysis method to examine the distribution of aggregation states of PDGF-beta receptors in human fibroblast cells. The method was able to resolve a tetrameric population of the PDGF-beta receptors relative to the background distribution of nonspecifically bound fluorophore.
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