ArticleGut2007
Effect of chronic Giardia lamblia infection on epithelial transport and barrier function in human duodenum.
Article in Gut, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 109 papers.
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Who cites it
109 citing papers in PubMed, 282 citations in OpenAlex.
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- Bile acid sequestration reveals microbiome remodeling that dominates FXR signaling to shape host responses to enteric infection.PLoS pathogens · 2026Article
- Influence of Cryptosporidium parvum and Giardia duodenalis on glucose transport mechanisms and tight junctions in co-infected enterocytes.Parasites & vectors · 2026Article
- Systemic inflammation and malaria co-infection in irritable bowel syndrome: a cross-sectional study of 142 Yemeni patients.BMJ open · 2026Observational
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- The differential roles of Giardia duodenalis-secreted tenascins in inducing intestinal epithelial cell apoptosis and the attributed EGFR-STAT3 axis regulation.PLoS neglected tropical diseases · 2026Article
- Interplay of Gastrointestinal Parasites, Micronutrient Deficiencies, and Anemia in Children from the Bolivian Highlands.Microorganisms · 2026Article
- Enteric Infections, Dysbiosis, and Metabolic Dysfunction: The Role of Diarrheagenic Pathogens in Insulin Resistance.International journal of molecular sciences · 2026Review
- A Trichinella spiralis serine protease triggers gut epithelial apoptosis and destroys the barrier integrity to mediate larval invasion.PLoS neglected tropical diseases · 2025Article
- Giardia-induced Type 2 mucosal immunity attenuates intestinal inflammation caused by co-infection or colitis in mice.Nature microbiology · 2025Article
- Establishment of an in vitro co-infection model of Cryptosporidium parvum and Giardia duodenalis.Parasites & vectors · 2025Article
- The Role ofPathogens (Basel, Switzerland) · 2025Review
- Trichinella spiralis excretory/secretory proteins mediated larval invasion via inducing gut epithelial apoptosis and barrier disruption.PLoS neglected tropical diseases · 2025Article
- Article
- Extracellular Cysteine Proteases of Key Intestinal Protozoan Pathogens-Factors Linked to Virulence and Pathogenicity.International journal of molecular sciences · 2023Review
- Intestinal Parasitic Infections in 2023.Gastroenterology research · 2023Review
- Ultrastructural changes and IgA modulatory effect of commercial prebiotic and probiotic in murine giardiasis.Journal of parasitic diseases : official organ of the Indian Society for Parasitology · 2023Article
- Trophozoite fitness dictates the intestinal epithelial cell response to Giardia intestinalis infection.PLoS pathogens · 2023Article
- Specific TLR-mediated HSP70 activation plays a potential role in host defense against the intestinal parasiteFrontiers in microbiology · 2023Article
49 more citing papers are in PubMed but not listed here.
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Authors and funding
10 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundGiardia lamblia causes infection of the small intestine, which leads to malabsorption and chronic diarrhoea.
aimTo characterise the inherent pathomechanisms of G lamblia infection.
methodsDuodenal biopsy specimens from 13 patients with chronic giardiasis and from controls were obtained endoscopically. Short-circuit current (I(SC)) and mannitol fluxes were measured in miniaturised Ussing chambers. Epithelial and subepithelial resistances were determined by impedance spectroscopy. Mucosal morphometry was performed and tight junction proteins were characterised by immunoblotting. Apoptotic ratio was determined by terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate nick-end labelling staining.
resultsIn giardiasis, mucosal surface area per unit serosa area was decreased to 75% (3%) of control, as a result of which epithelial resistance should increase. Instead, epithelial resistance of giardiasis biopsy specimens was decreased (19 (2) vs 25 (2) Omega cm(2); p<0.05) whereas mannitol flux was not significantly altered (140 (27) vs 105 (16) nmol/h/cm(2)). As structural correlate, reduced claudin 1 expression and increased epithelial apoptosis were detected. Furthermore, basal I(SC) increased from 191 (20) in control to 261 (12) microA/h/cm(2) in giardiasis. The bumetanide-sensitive portion of I(SC) in giardiasis was also increased (51 (5) vs 20 (9) microA/h/cm(2) in control; p<0.05). Finally, phlorizin-sensitive Na(+)-glucose symport was reduced in patients with giardiasis (121 (9) vs 83 (14) microA/h/cm(2)).
conclusionsG lamblia infection causes epithelial barrier dysfunction owing to down regulation of the tight junction protein claudin 1 and increased epithelial apoptoses. Na(+)-dependent d-glucose absorption is impaired and active electrogenic anion secretion is activated. Thus, the mechanisms of diarrhoea in human chronic giardiasis comprise leak flux, malabsorptive and secretory components.
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