ArticleMolecular and cellular biochemistry2007
Yeast holoenzyme of protein kinase CK2 requires both beta and beta' regulatory subunits for its activity.
Article in Molecular and cellular biochemistry, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers, 1 of them a synthesis that pooled it.
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18 citing papers in PubMed, 1 synthesis or guideline pooled it, 34 citations in OpenAlex.
- Predicting protein post-translational modifications using meta-analysis of proteome scale data sets.Molecular & cellular proteomics : MCP · 2009Pooled it
- Yeast Protein Asf1 Possesses Modulating Activity towards Protein Kinase CK2.International journal of molecular sciences · 2022Article
- A proteomic screen of Ty1 integrase partners identifies the protein kinase CK2 as a regulator of Ty1 retrotransposition.Mobile DNA · 2022Article
- Pharmacophore development, drug-likeness analysis, molecular docking, and molecular dynamics simulations for identification of new CK2 inhibitors.Journal of molecular modeling · 2020Article
- Inhibitory influence of natural flavonoids on human protein kinase CK2 isoforms: effect of the regulatory subunit.Molecular and cellular biochemistry · 2018Article
- The New Role for an Old Kinase: Protein Kinase CK2 Regulates Metal Ion Transport.Pharmaceuticals (Basel, Switzerland) · 2016Review
- Dual action antifungal small molecule modulates multidrug efflux and TOR signaling.Nature chemical biology · 2016Article
- Protein kinase CK2 holoenzyme promotes start-specific transcription in Saccharomyces cerevisiae.Eukaryotic cell · 2013Article
- Nutritional modulation of CK2 in Saccharomyces cerevisiae: regulating the activity of a constitutive enzyme.Molecular and cellular biochemistry · 2011Article
- Interactions between subunits of protein kinase CK2 and their protein substrates influences its sensitivity to specific inhibitors.Molecular and cellular biochemistry · 2011Article
- Identification of candidate genes related to bovine marbling using protein-protein interaction networks.International journal of biological sciences · 2011Article
- Suppression of Ycf1p function by Cka1p-dependent phosphorylation is attenuated in response to salt stress.FEMS yeast research · 2010Article
- Genome-wide screen in Saccharomyces cerevisiae identifies vacuolar protein sorting, autophagy, biosynthetic, and tRNA methylation genes involved in life span regulation.PLoS genetics · 2010Article
- Differential phosphorylation of plant translation initiation factors by Arabidopsis thaliana CK2 holoenzymes.The Journal of biological chemistry · 2009Article
- The emerging CK2 interactome: insights into the regulation and functions of CK2.Molecular and cellular biochemistry · 2008Article
- Yeast surviving factor Svf1 as a new interacting partner, regulator and in vitro substrate of protein kinase CK2.Molecular and cellular biochemistry · 2008Article
- Mechanisms regulating the protein kinases of Saccharomyces cerevisiae.Eukaryotic cell · 2007Review
- Regulation of pol III transcription by nutrient and stress signaling pathways.Biochimica et biophysica actaReview
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Protein kinase CK2 is a highly conserved Ser/Thr protein kinase that is ubiquitous among eucaryotic organisms and appears to play an important role in many cellular functions. This enzyme in yeast has a tetrameric structure composed of two catalytic (alpha and/or alpha') subunits and two regulatory beta and beta' subunits. Previously, we have reported isolation from yeast cells four active forms of CK2, composed of alphaalpha'betabeta', alpha2betabeta', alpha'2betabeta' and a free alpha'-catalytic subunit. Now, we report that in Saccharomyces cerevisiae CK2 holoenzyme regulatory beta subunit cannot substitute other beta' subunit and only both of them can form fully active enzymatic unit. We have examined the subunit composition of tetrameric complexes of yeast CK2 by transformation of yeast strains containing single deletion of the beta or beta' regulatory subunits with vectors carrying lacking CKB1 or CKB2 genes. CK2 holoenzyme activity was restored only in cases when both of them were present in the cell. Additional, co-immunoprecypitation experiments show that polyadenylation factor Fip1 interacts with catalytic alpha subunits of CK2 and interaction with beta subunits in the holoenzyme decreases CK2 activity towards this protein substrate. These data may help to elucidate the role of yeast protein kinase CK2beta/beta' subunits in the regulation of holoenzyme assembly and phosphotransferase activity.
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