ArticleJournal of industrial microbiology & biotechnology2007
Characterization of proteolytic bacteria from the Aleutian deep-sea and their proteases.
Article in Journal of industrial microbiology & biotechnology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 39 citations in OpenAlex.
- Redox and solvent-stable alkaline serine protease fromFrontiers in microbiology · 2025Article
- Anti-barnacle biofouling coatings for the protection of marine vessels: synthesis and progress.Environmental science and pollution research international · 2022Review
- Properties and Applications of Extremozymes from Deep-Sea Extremophilic Microorganisms: A Mini Review.Marine drugs · 2019Review
- Tripeptides From Casein Are Signal Molecules to Induce the Expression of the Extracellular Protease MCP-01 Gene in Marine BacteriumFrontiers in microbiology · 2019Article
- Depth-Resolved Variations of Cultivable Bacteria and Their Extracellular Enzymes in the Water Column of the New Britain Trench.Frontiers in microbiology · 2018Article
- Statistical optimization of fibrinolytic enzyme production by Pseudoalteromonas sp. IND11 using cow dung substrate by response surface methodology.SpringerPlus · 2014Article
- Biotechnology of cold-active proteases.Biology · 2013Article
- Article
- Diversity of both the cultivable protease-producing bacteria and their extracellular proteases in the sediments of the South China sea.Microbial ecology · 2009Article
- Molecular analysis of the gene encoding a cold-adapted halophilic subtilase from deep-sea psychrotolerant bacterium Pseudoalteromonas sp. SM9913: cloning, expression, characterization and function analysis of the C-terminal PPC domains.Extremophiles : life under extreme conditions · 2009Article
- Biofilm formation and proteolytic activities of Pseudoalteromonas bacteria that were isolated from fish farm sediments.Microbial biotechnology · 2009Article
- Isolation and characterization of metalloproteases with a novel domain structure by construction and screening of metagenomic libraries.Applied and environmental microbiology · 2009Article
- Genomics of deep-sea and sub-seafloor microbes.Microbial biotechnology · 2009Article
- Novel antifoulants: inhibition of larval attachment by proteases.Marine biotechnology (New York, N.Y.)Article
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Six deep-sea proteolytic bacteria taken from Aleutian margin sediments were screened; one of them produced a cold-adapted neutral halophilic protease. These bacteria belong to Pseudoalteromonas spp., which were identified by the 16S rDNA sequence. Of the six proteases produced, two were neutral cold-adapted proteases that showed their optimal activity at pH 7-8 and at temperature close to 35 degrees C, and the other four were alkaline proteases that showed their optimal activity at pH 9 and at temperature of 40-45 degrees C. The neutral cold-adapted protease E1 showed its optimal activity at a sodium chloride concentration of 2 M, whereas the activity of the other five proteases decreased at elevated sodium chloride concentrations. Protease E1 was purified to electrophoretic homogeneity and its molecular mass was 34 kDa, as estimated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). The molecular weight of protease E1 was determined to be 32,411 Da by mass spectrometric analysis. Phenylmethyl sulfonylfluoride (PMSF) did not inhibit the activity of this protease, whereas it was partially inhibited by ethylenediaminetetra-acetic acid sodium salt (EDTA-Na). De novo amino acid sequencing proved protease E1 to be a novel protein.
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