ArticleRNA (New York, N.Y.)2006
Analysis of the binding of the N-terminal conserved domain of yeast Cbf5p to a box H/ACA snoRNA.
Article in RNA (New York, N.Y.), 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 29 citations in OpenAlex.
- Mechanistic insight into the pseudouridylation of RNA.RNA biology · 2025Review
- SUMOylation- and GAR1-Dependent Regulation of Dyskerin Nuclear and Subnuclear Localization.Molecular and cellular biology · 2021Article
- The fungal snoRNAome.RNA (New York, N.Y.) · 2018Article
- Efficient RNA pseudouridylation by eukaryotic H/ACA ribonucleoproteins requires high affinity binding and correct positioning of guide RNA.Nucleic acids research · 2018Article
- Assembly and trafficking of box C/D and H/ACA snoRNPs.RNA biology · 2017Review
- Native gel electrophoresis of human telomerase distinguishes active complexes with or without dyskerin.Nucleic acids research · 2012Article
- The H/ACA RNP assembly factor SHQ1 functions as an RNA mimic.Genes & development · 2011Article
- The box H/ACA snoRNP assembly factor Shq1p is a chaperone protein homologous to Hsp90 cochaperones that binds to the Cbf5p enzyme.Journal of molecular biology · 2009Article
- SHQ1 is required prior to NAF1 for assembly of H/ACA small nucleolar and telomerase RNPs.RNA (New York, N.Y.) · 2009Article
- Different mechanisms for pseudouridine formation in yeast 5S and 5.8S rRNAs.Molecular and cellular biology · 2008Article
- The structure and function of small nucleolar ribonucleoproteins.Nucleic acids research · 2007Review
- Dynamic interactions within sub-complexes of the H/ACA pseudouridylation guide RNP.Nucleic acids research · 2007Article
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
During ribosome biogenesis, the RNA precursor to mature rRNAs undergoes numerous post-transcriptional chemical modifications of bases, including conversions of uridines to pseudouridines. In archaea and eukaryotes, these conversions are performed by box H/ACA small ribonucleoprotein particles (box H/ACA RNPs), which contain a small guide RNA responsible for the selection of substrate uridines and four proteins, including the pseudouridine synthase, Cbf5p. So far, no in vitro reconstitution of eukaryotic box H/ACA RNPs from purified components has been achieved, principally due to difficulties in purifying recombinant eukaryotic Cbf5p. In this study, we present the purification of a truncated derivative of yeast Cbf5p (Cbf5(Delta)p) that retains the highly conserved TRUB and PUA domains. We have used band retardation assays to show that Cbf5(Delta)p on its own binds to box H/ACA small nucleolar (sno)RNAs. We demonstrate that the conserved H and ACA boxes enhance the affinity of the protein for the snoRNA. Furthermore, like its archaeal homologs, Cbf5(Delta)p can bind to a single stem-loop-box ACA RNA. Finally, we report the first enzymatic footprinting analysis of a Cbf5-RNA complex. Our results are compatible with the view that two molecules of Cbf5p interact with a binding platform constituted by the 5' end of the RNA, the single-stranded hinge domain containing the conserved H box, and the 3' end of the molecule, including the conserved ACA box.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.